Chlorpromazine: specific inhibition of L-noradrenaline and 5-hydroxytryptamine uptake in synaptosomes from squid brain

Harvey B. Pollard; Jeffery L. Barker; Wilhelm A. Bohr; Michael J. Dowdall

doi:10.1016/0006-8993(75)90999-3

Chlorpromazine: specific inhibition of L-noradrenaline and 5-hydroxytryptamine uptake in synaptosomes from squid brain

Harvey B. Pollard^*
, Jeffery L. Barker
, Wilhelm A. Bohr
, Michael J. Dowdall

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

17 Scopus citations

Abstract

Synaptosomes derived from squid optic lobes were found to have specific, high affinity uptake systems for a variety of neurotransmitters. Chlorpromazine (CPZ) inhibited the uptake of l-noradrenaline (l-NA) and 5-hydroxytryptamine (5-HT) in a competitive fashion. (K_i = 1.7 × 10^-7 M and 2.5 × 10^-7 M, respectively.) By contrast, CPZ (10^-5 M) did not affect uptake of choline, aspartate, glutamate or glycine. These results suggest that permeation mechanisms for both l-NA 5-HT may have similar CPZ-sensitive sites.

Original language	English
Pages (from-to)	23-31
Number of pages	9
Journal	Brain Research
Volume	85
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(75)90999-3
State	Published - 21 Feb 1975

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title = "Chlorpromazine: specific inhibition of L-noradrenaline and 5-hydroxytryptamine uptake in synaptosomes from squid brain",

abstract = "Synaptosomes derived from squid optic lobes were found to have specific, high affinity uptake systems for a variety of neurotransmitters. Chlorpromazine (CPZ) inhibited the uptake of l-noradrenaline (l-NA) and 5-hydroxytryptamine (5-HT) in a competitive fashion. (Ki = 1.7 {\texttimes} 10-7 M and 2.5 {\texttimes} 10-7 M, respectively.) By contrast, CPZ (10-5 M) did not affect uptake of choline, aspartate, glutamate or glycine. These results suggest that permeation mechanisms for both l-NA 5-HT may have similar CPZ-sensitive sites.",

author = "Pollard, \{Harvey B.\} and Barker, \{Jeffery L.\} and Bohr, \{Wilhelm A.\} and Dowdall, \{Michael J.\}",

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doi = "10.1016/0006-8993(75)90999-3",

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T2 - specific inhibition of L-noradrenaline and 5-hydroxytryptamine uptake in synaptosomes from squid brain

AU - Pollard, Harvey B.

AU - Barker, Jeffery L.

AU - Bohr, Wilhelm A.

AU - Dowdall, Michael J.

PY - 1975/2/21

Y1 - 1975/2/21

N2 - Synaptosomes derived from squid optic lobes were found to have specific, high affinity uptake systems for a variety of neurotransmitters. Chlorpromazine (CPZ) inhibited the uptake of l-noradrenaline (l-NA) and 5-hydroxytryptamine (5-HT) in a competitive fashion. (Ki = 1.7 × 10-7 M and 2.5 × 10-7 M, respectively.) By contrast, CPZ (10-5 M) did not affect uptake of choline, aspartate, glutamate or glycine. These results suggest that permeation mechanisms for both l-NA 5-HT may have similar CPZ-sensitive sites.

AB - Synaptosomes derived from squid optic lobes were found to have specific, high affinity uptake systems for a variety of neurotransmitters. Chlorpromazine (CPZ) inhibited the uptake of l-noradrenaline (l-NA) and 5-hydroxytryptamine (5-HT) in a competitive fashion. (Ki = 1.7 × 10-7 M and 2.5 × 10-7 M, respectively.) By contrast, CPZ (10-5 M) did not affect uptake of choline, aspartate, glutamate or glycine. These results suggest that permeation mechanisms for both l-NA 5-HT may have similar CPZ-sensitive sites.

UR - https://www.scopus.com/pages/publications/0016434530

U2 - 10.1016/0006-8993(75)90999-3

DO - 10.1016/0006-8993(75)90999-3

M3 - Article

AN - SCOPUS:0016434530

SN - 0006-8993

VL - 85

SP - 23

EP - 31

JO - Brain Research

JF - Brain Research

IS - 1

ER -

Chlorpromazine: specific inhibition of L-noradrenaline and 5-hydroxytryptamine uptake in synaptosomes from squid brain

Abstract

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