TY - JOUR
T1 - Cloning and characterization of the Saccharomyces cerevisiae CDC6 gene
AU - Lisziewicz, Julianna
AU - Godany, Andrej
AU - Agoston, Denes V.
AU - Küntzel, Hans
PY - 1988/12/23
Y1 - 1988/12/23
N2 - The yeast cell division cycle gene CDC6 was isolated by complementation of a temperature-sensitive cdc6 mutant with a genomic library. The amino acid sequence of the 48 kDalton CDC6 gene product, as deduced from DNA sequence data, includes the three consensus peptide motifs involved in guanine nucleotide binding and GTPase activity, a target site for cAMP-dependent protein kinase and a carboxy-terminal domain related to metallothionine sequences. A plasmid-encoded CDC6-β-galactosidase hybrid protein was located at the plasma membrane by indirect immunofluorescence. Disruption experiments indicate that the CDC6 gene product is essential for mitotic growth.
AB - The yeast cell division cycle gene CDC6 was isolated by complementation of a temperature-sensitive cdc6 mutant with a genomic library. The amino acid sequence of the 48 kDalton CDC6 gene product, as deduced from DNA sequence data, includes the three consensus peptide motifs involved in guanine nucleotide binding and GTPase activity, a target site for cAMP-dependent protein kinase and a carboxy-terminal domain related to metallothionine sequences. A plasmid-encoded CDC6-β-galactosidase hybrid protein was located at the plasma membrane by indirect immunofluorescence. Disruption experiments indicate that the CDC6 gene product is essential for mitotic growth.
UR - http://www.scopus.com/inward/record.url?scp=0024229118&partnerID=8YFLogxK
U2 - 10.1093/nar/16.24.11507
DO - 10.1093/nar/16.24.11507
M3 - Article
C2 - 3062576
AN - SCOPUS:0024229118
SN - 0305-1048
VL - 16
SP - 11507
EP - 11520
JO - Nucleic Acids Research
JF - Nucleic Acids Research
IS - 24
ER -