Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice

David S. Pisetsky; Sandra A. Caster; Margaret Piper; David W. Scott; Alfred D. Steinberg

doi:10.1016/0008-8749(84)90074-1

Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice

David S. Pisetsky^*, Sandra A. Caster, Margaret Piper, David W. Scott, Alfred D. Steinberg

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

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Abstract

The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.

Original language	English
Pages (from-to)	32-40
Number of pages	9
Journal	Cellular Immunology
Volume	84
Issue number	1
DOIs	https://doi.org/10.1016/0008-8749(84)90074-1
State	Published - Mar 1984
Externally published	Yes

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@article{d83348d98f16448d882beafbc09fb622,

title = "Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice",

abstract = "The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.",

author = "Pisetsky, {David S.} and Caster, {Sandra A.} and Margaret Piper and Scott, {David W.} and Steinberg, {Alfred D.}",

note = "Funding Information: r Supported by the Medical Research Service, Veterans Administration, NIH Grant AM 27166, Basil O{\textquoteright}Connor Starter Research Grant 5-296 from the March of Dimes, and the Otis B. Parrish Research Fund. * Abbreviations used: xid, X-linked immunodeficiency gene of CBA/N mice; SRBCs, sheep red blood cells; LPS, lipopolysaccharide; ELISA, enzyme-linked immunosorbent assay;F l, fluorescein haptenic group.",

year = "1984",

month = mar,

doi = "10.1016/0008-8749(84)90074-1",

language = "English",

volume = "84",

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journal = "Cellular Immunology",

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T1 - Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice

AU - Pisetsky, David S.

AU - Caster, Sandra A.

AU - Piper, Margaret

AU - Scott, David W.

AU - Steinberg, Alfred D.

N1 - Funding Information: r Supported by the Medical Research Service, Veterans Administration, NIH Grant AM 27166, Basil O’Connor Starter Research Grant 5-296 from the March of Dimes, and the Otis B. Parrish Research Fund. * Abbreviations used: xid, X-linked immunodeficiency gene of CBA/N mice; SRBCs, sheep red blood cells; LPS, lipopolysaccharide; ELISA, enzyme-linked immunosorbent assay;F l, fluorescein haptenic group.

PY - 1984/3

Y1 - 1984/3

N2 - The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.

AB - The relationship between colony formation (cloning) of B cells and their activation in murine autoimmunity was investigated in MRL-lpr/lpr and MRL.xid mice. Cells from MRL-lpr/lpr mice showed similar requirements for in vitro growth as normal CBA/J and BALB/c cells, with maximal colony formation in the presence of the supporting factors lipopolysaccharide and sheep red blood cells. The frequency of colony-forming cells from MRL-lpr/lpr spleens or hapten-specific B-cell preparations was slightly greater than the two normal control strains, with this difference significant only for a comparison of BALB/c and MRL-lpr/lpr spleens. In contrast, MRL-lpr/lpr mice bearing the xid gene for B-cell immunodeficiency (MRL.xid) had markedly reduced B-cell colony formation. These mice nevertheless expressed anti-DNA antibodies, although at levels reduced from that of MRL-lpr/lpr controls. These results indicate that enhanced in vitro colony formation need not accompany B-cell hyperactivity in murine autoimmune disease and that autoantibody production can occur in mice with impairment in this growth property.

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Cloning of B cells from autoimmune MRL-lpr/lpr and MRL.xid mice

Abstract

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