Coamplified and overexpressed genes at ERBB2 locus in gastric cancer

Asta Varis; Alexander Zaika; Pauli Puolakkainen; Balint Nagy; Irene Madrigal; Arto Kokkola; Aki Väyrynen; Päivi Kärkkäinen; Christopher Moskaluk; Wa'el El-Rifai; Sakari Knuutila

doi:10.1002/ijc.20001

Coamplified and overexpressed genes at ERBB2 locus in gastric cancer

Asta Varis, Alexander Zaika, Pauli Puolakkainen, Balint Nagy, Irene Madrigal, Arto Kokkola, Aki Väyrynen, Päivi Kärkkäinen, Christopher Moskaluk, Wa'el El-Rifai, Sakari Knuutila^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

DNA copy number amplification at the chromosomal region of 17q is frequent in gastric cancer. Recently 17q21 was identified as the critical region for the amplicon formation because this region harbors the ERBB2 oncogene and several other targets, such as TOP2A and DARPP32. In our study, we characterized the amplification (52 cases) and expression (29 cases) levels of ERBB2, TOP2A and DARPP32 in gastric cancer samples. These 3 genes were concomitantly amplified in 17% of the intestinal type of gastric adenocarcinoma. However, the expression levels were independent, showing overexpression of DARPP32 (48%), TOP2A (17%) and ERBB2 (3%) studied by quantitative real-time PCR. The most frequently overexpressed gene, DARPP32, exhibited strong protein overexpression in 45% (30/66) of the cases in immunohistochemical study of gastric cancer tumor tissue array. Additional studies are required to thoroughly understand the biological significance of these genes in gastric cancer.

Original language	English
Pages (from-to)	548-553
Number of pages	6
Journal	International Journal of Cancer
Volume	109
Issue number	4
DOIs	https://doi.org/10.1002/ijc.20001
State	Published - 20 Apr 2004
Externally published	Yes

Keywords

DARPP32
ERBB2
Gastric cancer
Gene amplification
TOP2A

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title = "Coamplified and overexpressed genes at ERBB2 locus in gastric cancer",

abstract = "DNA copy number amplification at the chromosomal region of 17q is frequent in gastric cancer. Recently 17q21 was identified as the critical region for the amplicon formation because this region harbors the ERBB2 oncogene and several other targets, such as TOP2A and DARPP32. In our study, we characterized the amplification (52 cases) and expression (29 cases) levels of ERBB2, TOP2A and DARPP32 in gastric cancer samples. These 3 genes were concomitantly amplified in 17% of the intestinal type of gastric adenocarcinoma. However, the expression levels were independent, showing overexpression of DARPP32 (48%), TOP2A (17%) and ERBB2 (3%) studied by quantitative real-time PCR. The most frequently overexpressed gene, DARPP32, exhibited strong protein overexpression in 45% (30/66) of the cases in immunohistochemical study of gastric cancer tumor tissue array. Additional studies are required to thoroughly understand the biological significance of these genes in gastric cancer.",

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AU - Varis, Asta

AU - Zaika, Alexander

AU - Puolakkainen, Pauli

AU - Nagy, Balint

AU - Madrigal, Irene

AU - Kokkola, Arto

AU - Väyrynen, Aki

AU - Kärkkäinen, Päivi

AU - Moskaluk, Christopher

AU - El-Rifai, Wa'el

AU - Knuutila, Sakari

PY - 2004/4/20

Y1 - 2004/4/20

N2 - DNA copy number amplification at the chromosomal region of 17q is frequent in gastric cancer. Recently 17q21 was identified as the critical region for the amplicon formation because this region harbors the ERBB2 oncogene and several other targets, such as TOP2A and DARPP32. In our study, we characterized the amplification (52 cases) and expression (29 cases) levels of ERBB2, TOP2A and DARPP32 in gastric cancer samples. These 3 genes were concomitantly amplified in 17% of the intestinal type of gastric adenocarcinoma. However, the expression levels were independent, showing overexpression of DARPP32 (48%), TOP2A (17%) and ERBB2 (3%) studied by quantitative real-time PCR. The most frequently overexpressed gene, DARPP32, exhibited strong protein overexpression in 45% (30/66) of the cases in immunohistochemical study of gastric cancer tumor tissue array. Additional studies are required to thoroughly understand the biological significance of these genes in gastric cancer.

AB - DNA copy number amplification at the chromosomal region of 17q is frequent in gastric cancer. Recently 17q21 was identified as the critical region for the amplicon formation because this region harbors the ERBB2 oncogene and several other targets, such as TOP2A and DARPP32. In our study, we characterized the amplification (52 cases) and expression (29 cases) levels of ERBB2, TOP2A and DARPP32 in gastric cancer samples. These 3 genes were concomitantly amplified in 17% of the intestinal type of gastric adenocarcinoma. However, the expression levels were independent, showing overexpression of DARPP32 (48%), TOP2A (17%) and ERBB2 (3%) studied by quantitative real-time PCR. The most frequently overexpressed gene, DARPP32, exhibited strong protein overexpression in 45% (30/66) of the cases in immunohistochemical study of gastric cancer tumor tissue array. Additional studies are required to thoroughly understand the biological significance of these genes in gastric cancer.

KW - DARPP32

KW - ERBB2

KW - Gastric cancer

KW - Gene amplification

KW - TOP2A

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DO - 10.1002/ijc.20001

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SN - 0020-7136

VL - 109

SP - 548

EP - 553

JO - International Journal of Cancer

JF - International Journal of Cancer

IS - 4

ER -

Coamplified and overexpressed genes at ERBB2 locus in gastric cancer

Abstract

Keywords

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