Comparative electrophysiological properties of NG108-15 cells in serum-containing and serum-free media

V. C. Kowtha; J. N. Quong; H. J. Bryant; D. A. Stenger

doi:10.1016/0304-3940(93)90874-K

Comparative electrophysiological properties of NG108-15 cells in serum-containing and serum-free media

V. C. Kowtha, J. N. Quong, H. J. Bryant, D. A. Stenger^*

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

The electrophysiological properties of NG108-15 neuroblastoma × glioma hybrids were compared after culture in serum-containing medium (SCM) versus serum-free media (SFM) containing N2 or B27 supplements. The excitability of cells was media dependent (B27 > N2 > SCM). Action potential profiles of SFM cells were characterized by slower activation and prolonged after hyperpolarization which predisposed SFM cells to fire repetitively. The presence of three types of inward calcium currents was also revealed in SFM cells. These differential effects were primarily attributable to the media used with a secondary enhancement by the chemical differentiating agents used (dB-cAMP and forskolin).

Original language	English
Pages (from-to)	129-133
Number of pages	5
Journal	Neuroscience Letters
Volume	164
Issue number	1-2
DOIs	https://doi.org/10.1016/0304-3940(93)90874-K
State	Published - 24 Dec 1993

Keywords

Calcium channel
Excitability
NG108-15
Serum-free medium

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10.1016/0304-3940(93)90874-K

Cite this

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title = "Comparative electrophysiological properties of NG108-15 cells in serum-containing and serum-free media",

abstract = "The electrophysiological properties of NG108-15 neuroblastoma × glioma hybrids were compared after culture in serum-containing medium (SCM) versus serum-free media (SFM) containing N2 or B27 supplements. The excitability of cells was media dependent (B27 > N2 > SCM). Action potential profiles of SFM cells were characterized by slower activation and prolonged after hyperpolarization which predisposed SFM cells to fire repetitively. The presence of three types of inward calcium currents was also revealed in SFM cells. These differential effects were primarily attributable to the media used with a secondary enhancement by the chemical differentiating agents used (dB-cAMP and forskolin).",

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AU - Stenger, D. A.

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AB - The electrophysiological properties of NG108-15 neuroblastoma × glioma hybrids were compared after culture in serum-containing medium (SCM) versus serum-free media (SFM) containing N2 or B27 supplements. The excitability of cells was media dependent (B27 > N2 > SCM). Action potential profiles of SFM cells were characterized by slower activation and prolonged after hyperpolarization which predisposed SFM cells to fire repetitively. The presence of three types of inward calcium currents was also revealed in SFM cells. These differential effects were primarily attributable to the media used with a secondary enhancement by the chemical differentiating agents used (dB-cAMP and forskolin).

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