TY - JOUR
T1 - Comparative field evaluation of HIV rapid diagnostic assays using serum, urine, and oral mucosal transudate specimens
AU - Tribble, David R.
AU - Rodier, Guénaël R.
AU - Saad, Magdy D.
AU - Binson, Gérard
AU - Marrot, Fabrice
AU - Salah, Said
AU - Omar, Chakib
AU - Arthur, Ray R.
N1 - Funding Information:
The authors wish to thank Dr Neil Constantine for review and comments and the officials of the Djibouti Ministry of Health who strongly supported this study. Appreciation is extended to Murex, Norcross, GA, USA for supplying materials. This study was supported by the Naval Medical Research and Development Command, Naval Medical Command, National Capital Region, Bethesda, MD, 20814, USA, Work Unit No. 00101.KHX.3272.
PY - 1997/2
Y1 - 1997/2
N2 - Background: Comparative field utility of selected HIV-1 assays using homologous collections of serum, urine and oral mucosal transudate (OMT) was determined in adult populations from a tuberculosis hospital and STD clinic in Dijbouti, East Africa. Study design:Enzyme immunoassay with confirmatory Western blot was performed on all serum specimens for comparison with rapid, instrument-free assays (SUDS HIV-1, Murex; TestPack HIV-1/2, Abbot; and COMBAIDS HIV 1 + 2, SPAN Diagnostics) using various specimen sources. Delayed (48 h post-collection) testing was also performed on urine. Sensitivity and specificity for the rapid assays, in descending order, were as follows: serum SUDS HIV-1 assay (100%, 98.3%), serum COMBAIDS HIV-1/2 assay (98.4%, 99.6%), and OMT SUDS HIV-1 assay (98.4%, 94.5%). Results: The OMT EIA optical density cutoff value was modified resulting in an improved specificity from 89.1 to 99.6%; however, sensitivity decreased from 100 to 98.5%. Urine EIA and rapid assays demonstrated unacceptable test performance for use as a screening test.
AB - Background: Comparative field utility of selected HIV-1 assays using homologous collections of serum, urine and oral mucosal transudate (OMT) was determined in adult populations from a tuberculosis hospital and STD clinic in Dijbouti, East Africa. Study design:Enzyme immunoassay with confirmatory Western blot was performed on all serum specimens for comparison with rapid, instrument-free assays (SUDS HIV-1, Murex; TestPack HIV-1/2, Abbot; and COMBAIDS HIV 1 + 2, SPAN Diagnostics) using various specimen sources. Delayed (48 h post-collection) testing was also performed on urine. Sensitivity and specificity for the rapid assays, in descending order, were as follows: serum SUDS HIV-1 assay (100%, 98.3%), serum COMBAIDS HIV-1/2 assay (98.4%, 99.6%), and OMT SUDS HIV-1 assay (98.4%, 94.5%). Results: The OMT EIA optical density cutoff value was modified resulting in an improved specificity from 89.1 to 99.6%; however, sensitivity decreased from 100 to 98.5%. Urine EIA and rapid assays demonstrated unacceptable test performance for use as a screening test.
KW - AIDS
KW - Enzyme immunoassay
KW - HIV
KW - Oral mucosal transudate
KW - Rapid assay
KW - Urine
KW - Western blot
UR - http://www.scopus.com/inward/record.url?scp=0031080609&partnerID=8YFLogxK
U2 - 10.1016/S0928-0197(96)00261-9
DO - 10.1016/S0928-0197(96)00261-9
M3 - Article
C2 - 9126680
AN - SCOPUS:0031080609
SN - 0928-0197
VL - 7
SP - 127
EP - 132
JO - Clinical and Diagnostic Virology
JF - Clinical and Diagnostic Virology
IS - 3
ER -