Comparative field evaluation of HIV rapid diagnostic assays using serum, urine, and oral mucosal transudate specimens

David R. Tribble; Guénaël R. Rodier; Magdy D. Saad; Gérard Binson; Fabrice Marrot; Said Salah; Chakib Omar; Ray R. Arthur

doi:10.1016/S0928-0197(96)00261-9

Comparative field evaluation of HIV rapid diagnostic assays using serum, urine, and oral mucosal transudate specimens

David R. Tribble^*, Guénaël R. Rodier, Magdy D. Saad, Gérard Binson, Fabrice Marrot, Said Salah, Chakib Omar, Ray R. Arthur

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Background: Comparative field utility of selected HIV-1 assays using homologous collections of serum, urine and oral mucosal transudate (OMT) was determined in adult populations from a tuberculosis hospital and STD clinic in Dijbouti, East Africa. Study design:Enzyme immunoassay with confirmatory Western blot was performed on all serum specimens for comparison with rapid, instrument-free assays (SUDS HIV-1, Murex; TestPack HIV-1/2, Abbot; and COMBAIDS HIV 1 + 2, SPAN Diagnostics) using various specimen sources. Delayed (48 h post-collection) testing was also performed on urine. Sensitivity and specificity for the rapid assays, in descending order, were as follows: serum SUDS HIV-1 assay (100%, 98.3%), serum COMBAIDS HIV-1/2 assay (98.4%, 99.6%), and OMT SUDS HIV-1 assay (98.4%, 94.5%). Results: The OMT EIA optical density cutoff value was modified resulting in an improved specificity from 89.1 to 99.6%; however, sensitivity decreased from 100 to 98.5%. Urine EIA and rapid assays demonstrated unacceptable test performance for use as a screening test.

Original language	English
Pages (from-to)	127-132
Number of pages	6
Journal	Clinical and Diagnostic Virology
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/S0928-0197(96)00261-9
State	Published - Feb 1997
Externally published	Yes

Keywords

AIDS
Enzyme immunoassay
HIV
Oral mucosal transudate
Rapid assay
Urine
Western blot

Access to Document

10.1016/S0928-0197(96)00261-9

Cite this

@article{249abe875a654bfca875719b0577cda1,

title = "Comparative field evaluation of HIV rapid diagnostic assays using serum, urine, and oral mucosal transudate specimens",

abstract = "Background: Comparative field utility of selected HIV-1 assays using homologous collections of serum, urine and oral mucosal transudate (OMT) was determined in adult populations from a tuberculosis hospital and STD clinic in Dijbouti, East Africa. Study design:Enzyme immunoassay with confirmatory Western blot was performed on all serum specimens for comparison with rapid, instrument-free assays (SUDS HIV-1, Murex; TestPack HIV-1/2, Abbot; and COMBAIDS HIV 1 + 2, SPAN Diagnostics) using various specimen sources. Delayed (48 h post-collection) testing was also performed on urine. Sensitivity and specificity for the rapid assays, in descending order, were as follows: serum SUDS HIV-1 assay (100%, 98.3%), serum COMBAIDS HIV-1/2 assay (98.4%, 99.6%), and OMT SUDS HIV-1 assay (98.4%, 94.5%). Results: The OMT EIA optical density cutoff value was modified resulting in an improved specificity from 89.1 to 99.6%; however, sensitivity decreased from 100 to 98.5%. Urine EIA and rapid assays demonstrated unacceptable test performance for use as a screening test.",

keywords = "AIDS, Enzyme immunoassay, HIV, Oral mucosal transudate, Rapid assay, Urine, Western blot",

author = "Tribble, {David R.} and Rodier, {Gu{\'e}na{\"e}l R.} and Saad, {Magdy D.} and G{\'e}rard Binson and Fabrice Marrot and Said Salah and Chakib Omar and Arthur, {Ray R.}",

note = "Funding Information: The authors wish to thank Dr Neil Constantine for review and comments and the officials of the Djibouti Ministry of Health who strongly supported this study. Appreciation is extended to Murex, Norcross, GA, USA for supplying materials. This study was supported by the Naval Medical Research and Development Command, Naval Medical Command, National Capital Region, Bethesda, MD, 20814, USA, Work Unit No. 00101.KHX.3272.",

year = "1997",

month = feb,

doi = "10.1016/S0928-0197(96)00261-9",

language = "English",

volume = "7",

pages = "127--132",

journal = "Clinical and Diagnostic Virology",

issn = "0928-0197",

publisher = "Elsevier B.V.",

number = "3",

}

TY - JOUR

T1 - Comparative field evaluation of HIV rapid diagnostic assays using serum, urine, and oral mucosal transudate specimens

AU - Tribble, David R.

AU - Rodier, Guénaël R.

AU - Saad, Magdy D.

AU - Binson, Gérard

AU - Marrot, Fabrice

AU - Salah, Said

AU - Omar, Chakib

AU - Arthur, Ray R.

N1 - Funding Information: The authors wish to thank Dr Neil Constantine for review and comments and the officials of the Djibouti Ministry of Health who strongly supported this study. Appreciation is extended to Murex, Norcross, GA, USA for supplying materials. This study was supported by the Naval Medical Research and Development Command, Naval Medical Command, National Capital Region, Bethesda, MD, 20814, USA, Work Unit No. 00101.KHX.3272.

PY - 1997/2

Y1 - 1997/2

N2 - Background: Comparative field utility of selected HIV-1 assays using homologous collections of serum, urine and oral mucosal transudate (OMT) was determined in adult populations from a tuberculosis hospital and STD clinic in Dijbouti, East Africa. Study design:Enzyme immunoassay with confirmatory Western blot was performed on all serum specimens for comparison with rapid, instrument-free assays (SUDS HIV-1, Murex; TestPack HIV-1/2, Abbot; and COMBAIDS HIV 1 + 2, SPAN Diagnostics) using various specimen sources. Delayed (48 h post-collection) testing was also performed on urine. Sensitivity and specificity for the rapid assays, in descending order, were as follows: serum SUDS HIV-1 assay (100%, 98.3%), serum COMBAIDS HIV-1/2 assay (98.4%, 99.6%), and OMT SUDS HIV-1 assay (98.4%, 94.5%). Results: The OMT EIA optical density cutoff value was modified resulting in an improved specificity from 89.1 to 99.6%; however, sensitivity decreased from 100 to 98.5%. Urine EIA and rapid assays demonstrated unacceptable test performance for use as a screening test.

AB - Background: Comparative field utility of selected HIV-1 assays using homologous collections of serum, urine and oral mucosal transudate (OMT) was determined in adult populations from a tuberculosis hospital and STD clinic in Dijbouti, East Africa. Study design:Enzyme immunoassay with confirmatory Western blot was performed on all serum specimens for comparison with rapid, instrument-free assays (SUDS HIV-1, Murex; TestPack HIV-1/2, Abbot; and COMBAIDS HIV 1 + 2, SPAN Diagnostics) using various specimen sources. Delayed (48 h post-collection) testing was also performed on urine. Sensitivity and specificity for the rapid assays, in descending order, were as follows: serum SUDS HIV-1 assay (100%, 98.3%), serum COMBAIDS HIV-1/2 assay (98.4%, 99.6%), and OMT SUDS HIV-1 assay (98.4%, 94.5%). Results: The OMT EIA optical density cutoff value was modified resulting in an improved specificity from 89.1 to 99.6%; however, sensitivity decreased from 100 to 98.5%. Urine EIA and rapid assays demonstrated unacceptable test performance for use as a screening test.

KW - AIDS

KW - Enzyme immunoassay

KW - HIV

KW - Oral mucosal transudate

KW - Rapid assay

KW - Urine

KW - Western blot

UR - http://www.scopus.com/inward/record.url?scp=0031080609&partnerID=8YFLogxK

U2 - 10.1016/S0928-0197(96)00261-9

DO - 10.1016/S0928-0197(96)00261-9

M3 - Article

C2 - 9126680

AN - SCOPUS:0031080609

SN - 0928-0197

VL - 7

SP - 127

EP - 132

JO - Clinical and Diagnostic Virology

JF - Clinical and Diagnostic Virology

IS - 3

ER -

Comparative field evaluation of HIV rapid diagnostic assays using serum, urine, and oral mucosal transudate specimens

Abstract

Keywords

Access to Document

Fingerprint

Cite this