TY - JOUR
T1 - Comparison of platelet quality and function across apheresis collection platforms
AU - Thomas, Kimberly A.
AU - Srinivasan, Amudan J.
AU - McIntosh, Colby
AU - Rahn, Katelin
AU - Kelly, Scott
AU - McGough, Lilian
AU - Clayton, Skye
AU - Perez, Samantha
AU - Smith, Alexandra
AU - Vavro, Lisa
AU - Musgrove, Javonn
AU - Hill, Ronnie
AU - Mdaki, Kennedy S.
AU - Bynum, James A.
AU - Meledeo, M. Adam
AU - Cap, Andrew P.
AU - Spinella, Philip C.
AU - Reddoch-Cardenas, Kristin M.
AU - Shea, Susan M.
N1 - Publisher Copyright:
© 2023 AABB.
PY - 2023/5
Y1 - 2023/5
N2 - Background: Platelet concentrates (PLT) can be manufactured using a combination of apheresis collection devices and suspension media (plasma or platelet additive solution (PAS)). It is unclear how platelet quality and hemostatic function differ across the current in-use manufacturing methods in the United States. The objective of this study was therefore to compare baseline function of PLT collected using different apheresis collection platforms and storage media. Study Design and Methods: PLT were collected at two sites with identical protocols (N = 5 per site, N = 10 total per group) on the MCS® + 9000 (Haemonetics; “MCS”), the Trima Accel® 7 (Terumo; “Trima”), and the Amicus Cell Separator (Fresenius Kabi, “Amicus”). MCS PLT were collected into plasma while Trima and Amicus PLT were collected into plasma or PAS (Trima into Isoplate and Amicus into InterSol; yielding groups “TP”, “TI” and “AP”, “AI”, respectively). PLT units were sampled 1 h after collection and assayed to compare cellular counts, biochemistry, and hemostatic function. Results: Differences in biochemistry were most evident between plasma and PAS groups, as anticipated. MCS and TP had the highest clot strength as assessed by viscoelastometry. AI had the lowest thrombin generation capacity. Both TP and TI had the highest responses on platelet aggregometry. AI had the greatest number of microparticles. Discussion: Platelet quality and function differ among collection platforms at baseline. MCS and Trima platelets overall appear to trend toward higher hemostatic function. Future investigations will assess how these differences change throughout storage, and if these in vitro measures are clinically relevant.
AB - Background: Platelet concentrates (PLT) can be manufactured using a combination of apheresis collection devices and suspension media (plasma or platelet additive solution (PAS)). It is unclear how platelet quality and hemostatic function differ across the current in-use manufacturing methods in the United States. The objective of this study was therefore to compare baseline function of PLT collected using different apheresis collection platforms and storage media. Study Design and Methods: PLT were collected at two sites with identical protocols (N = 5 per site, N = 10 total per group) on the MCS® + 9000 (Haemonetics; “MCS”), the Trima Accel® 7 (Terumo; “Trima”), and the Amicus Cell Separator (Fresenius Kabi, “Amicus”). MCS PLT were collected into plasma while Trima and Amicus PLT were collected into plasma or PAS (Trima into Isoplate and Amicus into InterSol; yielding groups “TP”, “TI” and “AP”, “AI”, respectively). PLT units were sampled 1 h after collection and assayed to compare cellular counts, biochemistry, and hemostatic function. Results: Differences in biochemistry were most evident between plasma and PAS groups, as anticipated. MCS and TP had the highest clot strength as assessed by viscoelastometry. AI had the lowest thrombin generation capacity. Both TP and TI had the highest responses on platelet aggregometry. AI had the greatest number of microparticles. Discussion: Platelet quality and function differ among collection platforms at baseline. MCS and Trima platelets overall appear to trend toward higher hemostatic function. Future investigations will assess how these differences change throughout storage, and if these in vitro measures are clinically relevant.
UR - http://www.scopus.com/inward/record.url?scp=85154560103&partnerID=8YFLogxK
U2 - 10.1111/trf.17370
DO - 10.1111/trf.17370
M3 - Article
C2 - 37070399
AN - SCOPUS:85154560103
SN - 0041-1132
VL - 63
SP - S146-S158
JO - Transfusion
JF - Transfusion
IS - S3
ER -