Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

David A. Doron; Richard M. McCarron; Eliahu Heldman; Anna Leena Sirén; Maria Spatz; Giora Feuerstein; Harvey B. Pollard; John M. Hallenbeck

doi:10.1016/0006-8993(92)91493-X

Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

David A. Doron
, Richard M. McCarron
, Eliahu Heldman
, Anna Leena Sirén
, Maria Spatz
, Giora Feuerstein
, Harvey B. Pollard
, John M. Hallenbeck^*

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca²⁺-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

Original language	English
Pages (from-to)	346-349
Number of pages	4
Journal	Brain Research
Volume	597
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(92)91493-X
State	Published - 4 Dec 1992

Keywords

Endothelium
Lipopolysaccharide
Thromboplastin

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title = "Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats",

abstract = "The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.",

keywords = "Endothelium, Lipopolysaccharide, Thromboplastin",

author = "Doron, \{David A.\} and McCarron, \{Richard M.\} and Eliahu Heldman and Sir{\'e}n, \{Anna Leena\} and Maria Spatz and Giora Feuerstein and Pollard, \{Harvey B.\} and Hallenbeck, \{John M.\}",

year = "1992",

month = dec,

day = "4",

doi = "10.1016/0006-8993(92)91493-X",

language = "English",

volume = "597",

pages = "346--349",

journal = "Brain Research",

issn = "0006-8993",

publisher = "Elsevier B.V.",

number = "2",

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TY - JOUR

T1 - Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

AU - Doron, David A.

AU - McCarron, Richard M.

AU - Heldman, Eliahu

AU - Sirén, Anna Leena

AU - Spatz, Maria

AU - Feuerstein, Giora

AU - Pollard, Harvey B.

AU - Hallenbeck, John M.

PY - 1992/12/4

Y1 - 1992/12/4

N2 - The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

AB - The amounts of tissue factor (TF) expressed by brain microvascular endothelial cells (BMECs) from normotensive Wistar-Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were compared after stimulating the cells with different doses of lipopolysaccharide (LPS), thrombin, phorbol myristic acid (PMA), Ca2+-ionophore (A23187), or tumor necrosis factor (TNF) and interleukin-1 (IL-1). Treatment of cultured BMECs from WKY and SHR with all of these factors dose-dependently increased their total amount of TF; no substantive differences in the levels of enhanced TF expression were observed between WKY and SHR BMECs. We conclude that stimulated endothelium from rats with hypertension, a major stroke risk factor, is not hyperresponsive with respect to TF expression when compared to normotensive controls.

KW - Endothelium

KW - Lipopolysaccharide

KW - Thromboplastin

UR - https://www.scopus.com/pages/publications/0026747245

U2 - 10.1016/0006-8993(92)91493-X

DO - 10.1016/0006-8993(92)91493-X

M3 - Article

C2 - 1473006

AN - SCOPUS:0026747245

SN - 0006-8993

VL - 597

SP - 346

EP - 349

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Comparison of stimulated tissue factor expression by brain microvascular endothelial cells from normotensive (WKY) and hypertensive (SHR) rats

Abstract

Keywords

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