Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory

Eszter Deak; Carmen L. Charlton; April M. Bobenchik; Shelley A. Miller; Simon Pollett; Ian H. McHardy; Max T. Wu; Omai B. Garner

doi:10.1016/j.diagmicrobio.2014.09.018

Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory

Eszter Deak, Carmen L. Charlton, April M. Bobenchik, Shelley A. Miller, Simon Pollett, Ian H. McHardy, Max T. Wu, Omai B. Garner^*

^*Corresponding author for this work

Infectious Disease Clinical Research Program (IDCRP)

Research output: Contribution to journal › Article › peer-review

66 Scopus citations

Abstract

This study compared the diagnostic performance of Bruker's Microflex LT and bioMérieux's Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. A total of 477 isolates were tested on both instruments. Discrepant results were resolved by sequencing. Overall, there was no statistically significant difference between the proportion of isolates correctly identified, miscalled or not called by each instrument. Although both systems were good at identifying yeast (66/69 to species level), the confidence level was high only to genus level for 30% of the isolates on the Bruker. Both systems performed with high accuracy when evaluated solely on Food and Drug Administration-approved organisms for each database. A user-based assessment of the 2 instruments revealed an overall preference for the Vitek MS instrument.

Original language	English
Pages (from-to)	27-33
Number of pages	7
Journal	Diagnostic Microbiology and Infectious Disease
Volume	81
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2014.09.018
State	Published - 1 Jan 2015

Keywords

Bacteriology
Clinical microbiology
MALDI-TOF
Mycology
Rapid identification

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Deak, E., Charlton, C. L., Bobenchik, A. M., Miller, S. A., Pollett, S., McHardy, I. H., Wu, M. T., & Garner, O. B. (2015). Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory. Diagnostic Microbiology and Infectious Disease, 81(1), 27-33. https://doi.org/10.1016/j.diagmicrobio.2014.09.018

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title = "Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory",

abstract = "This study compared the diagnostic performance of Bruker's Microflex LT and bioM{\'e}rieux's Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. A total of 477 isolates were tested on both instruments. Discrepant results were resolved by sequencing. Overall, there was no statistically significant difference between the proportion of isolates correctly identified, miscalled or not called by each instrument. Although both systems were good at identifying yeast (66/69 to species level), the confidence level was high only to genus level for 30% of the isolates on the Bruker. Both systems performed with high accuracy when evaluated solely on Food and Drug Administration-approved organisms for each database. A user-based assessment of the 2 instruments revealed an overall preference for the Vitek MS instrument.",

keywords = "Bacteriology, Clinical microbiology, MALDI-TOF, Mycology, Rapid identification",

author = "Eszter Deak and Charlton, {Carmen L.} and Bobenchik, {April M.} and Miller, {Shelley A.} and Simon Pollett and McHardy, {Ian H.} and Wu, {Max T.} and Garner, {Omai B.}",

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Deak, E, Charlton, CL, Bobenchik, AM, Miller, SA, Pollett, S, McHardy, IH, Wu, MT & Garner, OB 2015, 'Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory', Diagnostic Microbiology and Infectious Disease, vol. 81, no. 1, pp. 27-33. https://doi.org/10.1016/j.diagmicrobio.2014.09.018

Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory. / Deak, Eszter; Charlton, Carmen L.; Bobenchik, April M. et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 81, No. 1, 01.01.2015, p. 27-33.

Research output: Contribution to journal › Article › peer-review

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AU - Charlton, Carmen L.

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AU - Miller, Shelley A.

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AB - This study compared the diagnostic performance of Bruker's Microflex LT and bioMérieux's Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems. A total of 477 isolates were tested on both instruments. Discrepant results were resolved by sequencing. Overall, there was no statistically significant difference between the proportion of isolates correctly identified, miscalled or not called by each instrument. Although both systems were good at identifying yeast (66/69 to species level), the confidence level was high only to genus level for 30% of the isolates on the Bruker. Both systems performed with high accuracy when evaluated solely on Food and Drug Administration-approved organisms for each database. A user-based assessment of the 2 instruments revealed an overall preference for the Vitek MS instrument.

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KW - Mycology

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Comparison of the Vitek MS and Bruker Microflex LT MALDI-TOF MS platforms for routine identification of commonly isolated bacteria and yeast in the clinical microbiology laboratory

Abstract

Keywords

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