Composite ECM-alginate microfibers produced by microfluidics as scaffolds with biomineralization potential

Marco Angelozzi; Martina Miotto; Letizia Penolazzi; Stefania Mazzitelli; Timothy Keane; Stephen F. Badylak; Roberta Piva; Claudio Nastruzzi

doi:10.1016/j.msec.2015.06.004

Composite ECM-alginate microfibers produced by microfluidics as scaffolds with biomineralization potential

Marco Angelozzi, Martina Miotto, Letizia Penolazzi, Stefania Mazzitelli, Timothy Keane, Stephen F. Badylak, Roberta Piva^*, Claudio Nastruzzi

^*Corresponding author for this work

Surgery

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

A novel approach to produce artificial bone composites (microfibers) with distinctive features mimicking natural tissue was investigated. Currently proposed inorganic materials (e.g. apatite matrixes) lack self-assembly and thereby limit interactions between cells and the material. The present work investigates the feasibility of creating "bio-inspired materials" specifically designed to overcome certain limitations inherent to current biomaterials. We examined the dimensions, morphology, and constitutive features of a composite hydrogel which combined an alginate based microfiber with a gelatin solution or a particulate form of urinary bladder matrix (UBM). The effectiveness of the composite microfibers to induce and modulate osteoblastic differentiation in three-dimensional (3D) scaffolds without altering the viability and morphological characteristics of the cells was investigated. The present study describes a novel alginate microfiber production method with the use of microfluidics. The microfluidic procedure allowed for precise tuning of microfibers which resulted in enhanced viability and function of embedded cells.

Original language	English
Pages (from-to)	141-153
Number of pages	13
Journal	Materials Science and Engineering C
Volume	56
DOIs	https://doi.org/10.1016/j.msec.2015.06.004
State	Published - 20 Jun 2015

Keywords

Alginate
Microfibers
Microfluidics
Osteoblastic differentiation
UBM

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10.1016/j.msec.2015.06.004

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title = "Composite ECM-alginate microfibers produced by microfluidics as scaffolds with biomineralization potential",

abstract = "A novel approach to produce artificial bone composites (microfibers) with distinctive features mimicking natural tissue was investigated. Currently proposed inorganic materials (e.g. apatite matrixes) lack self-assembly and thereby limit interactions between cells and the material. The present work investigates the feasibility of creating {"}bio-inspired materials{"} specifically designed to overcome certain limitations inherent to current biomaterials. We examined the dimensions, morphology, and constitutive features of a composite hydrogel which combined an alginate based microfiber with a gelatin solution or a particulate form of urinary bladder matrix (UBM). The effectiveness of the composite microfibers to induce and modulate osteoblastic differentiation in three-dimensional (3D) scaffolds without altering the viability and morphological characteristics of the cells was investigated. The present study describes a novel alginate microfiber production method with the use of microfluidics. The microfluidic procedure allowed for precise tuning of microfibers which resulted in enhanced viability and function of embedded cells.",

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AU - Angelozzi, Marco

AU - Miotto, Martina

AU - Penolazzi, Letizia

AU - Mazzitelli, Stefania

AU - Keane, Timothy

AU - Badylak, Stephen F.

AU - Piva, Roberta

AU - Nastruzzi, Claudio

PY - 2015/6/20

Y1 - 2015/6/20

N2 - A novel approach to produce artificial bone composites (microfibers) with distinctive features mimicking natural tissue was investigated. Currently proposed inorganic materials (e.g. apatite matrixes) lack self-assembly and thereby limit interactions between cells and the material. The present work investigates the feasibility of creating "bio-inspired materials" specifically designed to overcome certain limitations inherent to current biomaterials. We examined the dimensions, morphology, and constitutive features of a composite hydrogel which combined an alginate based microfiber with a gelatin solution or a particulate form of urinary bladder matrix (UBM). The effectiveness of the composite microfibers to induce and modulate osteoblastic differentiation in three-dimensional (3D) scaffolds without altering the viability and morphological characteristics of the cells was investigated. The present study describes a novel alginate microfiber production method with the use of microfluidics. The microfluidic procedure allowed for precise tuning of microfibers which resulted in enhanced viability and function of embedded cells.

AB - A novel approach to produce artificial bone composites (microfibers) with distinctive features mimicking natural tissue was investigated. Currently proposed inorganic materials (e.g. apatite matrixes) lack self-assembly and thereby limit interactions between cells and the material. The present work investigates the feasibility of creating "bio-inspired materials" specifically designed to overcome certain limitations inherent to current biomaterials. We examined the dimensions, morphology, and constitutive features of a composite hydrogel which combined an alginate based microfiber with a gelatin solution or a particulate form of urinary bladder matrix (UBM). The effectiveness of the composite microfibers to induce and modulate osteoblastic differentiation in three-dimensional (3D) scaffolds without altering the viability and morphological characteristics of the cells was investigated. The present study describes a novel alginate microfiber production method with the use of microfluidics. The microfluidic procedure allowed for precise tuning of microfibers which resulted in enhanced viability and function of embedded cells.

KW - Alginate

KW - Microfibers

KW - Microfluidics

KW - Osteoblastic differentiation

KW - UBM

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EP - 153

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

ER -

Composite ECM-alginate microfibers produced by microfluidics as scaffolds with biomineralization potential

Abstract

Keywords

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