Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts

Himaja Gaddipati; Marina V Pryzhkova; Philip W Jordan

doi:10.1007/978-1-4939-9520-2_4

Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts

Himaja Gaddipati, Marina V Pryzhkova, Philip W Jordan

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.

Original language	English
Pages (from-to)	35-46
Number of pages	12
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	2004
DOIs	https://doi.org/10.1007/978-1-4939-9520-2_4
State	Published - 2019
Externally published	Yes

Keywords

Animals
Cell Cycle Proteins/genetics
Cell Line
Chromosomes/genetics
Fibroblasts/physiology
Genome/genetics
Mice
Mice, Knockout
Mutation/genetics
NIH 3T3 Cells
RNA Interference/physiology

Access to Document

10.1007/978-1-4939-9520-2_4

Cite this

@article{c78d73e15ba54112bbbc31b689b8367e,

title = "Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts",

abstract = "The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.",

keywords = "Animals, Cell Cycle Proteins/genetics, Cell Line, Chromosomes/genetics, Fibroblasts/physiology, Genome/genetics, Mice, Mice, Knockout, Mutation/genetics, NIH 3T3 Cells, RNA Interference/physiology",

author = "Himaja Gaddipati and Pryzhkova, {Marina V} and Jordan, {Philip W}",

year = "2019",

doi = "10.1007/978-1-4939-9520-2_4",

language = "English",

volume = "2004",

pages = "35--46",

journal = "Methods in molecular biology (Clifton, N.J.)",

issn = "1064-3745",

}

TY - JOUR

T1 - Conditional Mutation of SMC5 in Mouse Embryonic Fibroblasts

AU - Gaddipati, Himaja

AU - Pryzhkova, Marina V

AU - Jordan, Philip W

PY - 2019

Y1 - 2019

N2 - The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.

AB - The structural maintenance of chromosomes (SMC) complex, SMC5/6, is important for genome maintenance in all model eukaryotes. To date, the most extensive studies have focused on the roles of Smc5/6 in lower eukaryotes, such as yeast and fly. In the handful of studies that have used mammalian cells, siRNA was used by most to knockdown SMC5/6 components. RNAi methods have been very important for scientific progression, but they are hindered by incomplete silencing of protein expression and off-target effects. This chapter outlines the use of a conditional knockout approach in mouse embryonic fibroblasts to study the function of the SMC5/6 complex. These cell lines provide an alternative method to study the function and properties of the SMC5/6 complex in mammals.

KW - Animals

KW - Cell Cycle Proteins/genetics

KW - Cell Line

KW - Chromosomes/genetics

KW - Fibroblasts/physiology

KW - Genome/genetics

KW - Mice

KW - Mice, Knockout

KW - Mutation/genetics

KW - NIH 3T3 Cells

KW - RNA Interference/physiology

U2 - 10.1007/978-1-4939-9520-2_4

DO - 10.1007/978-1-4939-9520-2_4

M3 - Article

C2 - 31147908

SN - 1064-3745

VL - 2004

SP - 35

EP - 46

JO - Methods in molecular biology (Clifton, N.J.)

JF - Methods in molecular biology (Clifton, N.J.)

ER -