Construction of a three-dimensional iso-density map of the low-density lipoprotein particle from human serum

Harvey B. Pollard; Sumana K. Devi

doi:10.1016/S0006-291X(71)80124-9

Construction of a three-dimensional iso-density map of the low-density lipoprotein particle from human serum

Harvey B. Pollard^*, Sumana K. Devi

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A three-dimensional iso-density map of negatively stained low density lipoprotein (LDL) from human serum has been constructed. Iso-density data were collected from electron micrographs of LDL by scanning at one-micron intervals with a Tech/Ops Isodensitracer. Isodensity contour data were plotted directly on X-Y coordinates. A direct 50-fold enlargement of the particle was achieved with a significant reduction in the signal to noise ratio when compared to conventional enlargement methods. The best three-dimensional distribution of the isodensity data was that of a pentagonal-dodecahedron with isodensity maxima at the vertices. Based on this and other studies (1) these maxima were thought to represent protein subunits.

Original language	English
Pages (from-to)	593-599
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	44
Issue number	3
DOIs	https://doi.org/10.1016/S0006-291X(71)80124-9
State	Published - 6 Aug 1971

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10.1016/S0006-291X(71)80124-9

Cite this

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title = "Construction of a three-dimensional iso-density map of the low-density lipoprotein particle from human serum",

abstract = "A three-dimensional iso-density map of negatively stained low density lipoprotein (LDL) from human serum has been constructed. Iso-density data were collected from electron micrographs of LDL by scanning at one-micron intervals with a Tech/Ops Isodensitracer. Isodensity contour data were plotted directly on X-Y coordinates. A direct 50-fold enlargement of the particle was achieved with a significant reduction in the signal to noise ratio when compared to conventional enlargement methods. The best three-dimensional distribution of the isodensity data was that of a pentagonal-dodecahedron with isodensity maxima at the vertices. Based on this and other studies (1) these maxima were thought to represent protein subunits.",

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T1 - Construction of a three-dimensional iso-density map of the low-density lipoprotein particle from human serum

AU - Pollard, Harvey B.

AU - Devi, Sumana K.

PY - 1971/8/6

Y1 - 1971/8/6

N2 - A three-dimensional iso-density map of negatively stained low density lipoprotein (LDL) from human serum has been constructed. Iso-density data were collected from electron micrographs of LDL by scanning at one-micron intervals with a Tech/Ops Isodensitracer. Isodensity contour data were plotted directly on X-Y coordinates. A direct 50-fold enlargement of the particle was achieved with a significant reduction in the signal to noise ratio when compared to conventional enlargement methods. The best three-dimensional distribution of the isodensity data was that of a pentagonal-dodecahedron with isodensity maxima at the vertices. Based on this and other studies (1) these maxima were thought to represent protein subunits.

AB - A three-dimensional iso-density map of negatively stained low density lipoprotein (LDL) from human serum has been constructed. Iso-density data were collected from electron micrographs of LDL by scanning at one-micron intervals with a Tech/Ops Isodensitracer. Isodensity contour data were plotted directly on X-Y coordinates. A direct 50-fold enlargement of the particle was achieved with a significant reduction in the signal to noise ratio when compared to conventional enlargement methods. The best three-dimensional distribution of the isodensity data was that of a pentagonal-dodecahedron with isodensity maxima at the vertices. Based on this and other studies (1) these maxima were thought to represent protein subunits.

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