Correlation of clinical data with fallopian tube specimen immune cells and tissue culture capacity

Satish Kumar Ramraj, Katie M. Smith, Naveena B. Janakiram, Coralee Toal, Ankita Raman, Doris Mangiaracina Benbrook*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

Human fallopian tube fimbria secretory epithelial cells (hFTSECs) are considered an origin of ovarian cancer and methods for their culture from fallopian tube specimens have been reported. Our objective was to determine whether characteristics of the donors or surgeries were associated with the capacities of fimbria specimens to generate hFTSEC cultures or their immune profiles. There were no surgical complications attributable to fallopian tube removal. Attempts to establish primary hFTSEC cultures were successful in 37 of 55 specimens (67%). Success rates did not differ significantly between specimens grouped by patient or surgery characteristics. Established cultures could be revived after cryopreservation and none became contaminated with microorganisms. Two cultures evaluated for long term growth senesced between passages 10 and 15. M1 macrophages were the predominant cell type, while all other immune cells were present at much lower percentages. IL-10 and TGF-β exhibited opposing trends with M1 and M2 macrophages. Plasma IL-10 levels exhibited significant positive correlation with patient age. In conclusion, fallopian tube fimbria specimens exhibit a pro-inflammatory phenotype and can be used to provide a source of hFTSECs that can be cultured for a limited time regardless of the donor patient age or race, or the type of surgery performed.

Original languageEnglish
Pages (from-to)57-64
Number of pages8
JournalTissue and Cell
Volume52
DOIs
StatePublished - Jun 2018
Externally publishedYes

Keywords

  • Cytokines
  • Fallopian tube fimbria
  • Human fallopian tube secretory epithelial cell cultures
  • Infiltrating immune cells
  • Salpingectomy

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