Costimulation blockade alters germinal center responses and prevents antibody-mediated rejection

E. J. Kim, J. Kwun, A. C. Gibby, J. J. Hong, A. B. Farris, N. N. Iwakoshi, F. Villinger, A. D. Kirk, S. J. Knechtle*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

153 Scopus citations

Abstract

De novo donor-specific antibody (DSA) after organ transplantation promotes antibody-mediated rejection (AMR) and causes late graft loss. Previously, we demonstrated that depletion using anti-CD3 immunotoxin combined with tacrolimus and alefacept (AMR regimen) reliably induced early DSA production with AMR in a nonhuman primate kidney transplant model. Five animals were assigned as positive AMR controls, four received additional belatacept and four received additional anti-CD40 mAb (2C10R4). Notably, production of early de novo DSA was completely attenuated with additional belatacept or 2C10R4 treatment. In accordance with this, while positive controls experienced a decrease in peripheral IgM + B cells, bela- and 2C10R4-added groups maintained a predominant population of IgM+ B cells, potentially indicating decreased isotype switching. Central memory T cells (CD4+CD28+CD95 +) as well as PD-1hiCD4+ T cells were decreased in both bela-added and 2C10R4-added groups. In analyzing germinal center (GC) reactions in situ, lymph nodes further revealed a reduction of B cell clonal expansion, GC-follicular helper T (Tfh) cells, and IL-21 production inside GCs with additional belatacept or 2C10R4 treatment. Here we provide evidence that belatacept and 2C10R4 selectively suppresses the humoral response via regulating Tfh cells and prevents AMR in this nonhuman primate model. Costimulation blockade with belatacept or the CD40-specific antibody 2C10R4 selectively suppresses the humoral response by regulating follicular helper T cells, and prevents antibody-mediated rejection in a T cell depletion-based nonhuman primate renal transplant model.

Original languageEnglish
Pages (from-to)59-69
Number of pages11
JournalAmerican Journal of Transplantation
Volume14
Issue number1
DOIs
StatePublished - Jan 2014
Externally publishedYes

Keywords

  • Antibody-mediated rejection
  • costimulation blockade
  • follicular helper T cells
  • germinal center reaction

Fingerprint

Dive into the research topics of 'Costimulation blockade alters germinal center responses and prevents antibody-mediated rejection'. Together they form a unique fingerprint.

Cite this