Cross-clade neutralization patterns among HIV-1 strains from the six major clades of the pandemic evaluated and compared in two different models

Bruce K. Brown; Lindsay Wieczorek; Eric Sanders-Buell; Andrew Rosa Borges; Merlin L. Robb; Deborah L. Birx; Nelson L. Michael; Francine E. McCutchan; Victoria R. Polonis

doi:10.1016/j.virol.2008.02.022

Cross-clade neutralization patterns among HIV-1 strains from the six major clades of the pandemic evaluated and compared in two different models

Bruce K. Brown, Lindsay Wieczorek, Eric Sanders-Buell, Andrew Rosa Borges, Merlin L. Robb, Deborah L. Birx, Nelson L. Michael, Francine E. McCutchan, Victoria R. Polonis^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

81 Scopus citations

Abstract

A panel of paired primary virus isolates and envelope pseudoviruses from sixty strains representing six HIV-1 clades was tested for neutralization using pooled, clade-specific plasma in two prominently utilized neutralization platforms: a primary isolate assay using peripheral blood mononuclear cells (PBMC) and a pseudovirus assay using a reporter epithelial cell line. Using the PMBC assay, pairing of the antibody pool against homologous clade viruses generated the highest geometric mean neutralizing antibody titer in 4 out of 6 clades tested, and neutralization patterns showed numerous examples of reciprocal cross-recognition between antibody and viruses of specific clade pairs. In the pseudovirus assay, cross-clade neutralization was more limited, with fewer distinct cross-clade relationships evident. The clade C antibody pool was broadly cross-reactive, neutralizing the greatest number of viruses in both assays. These data highlight the importance of the neutralization assay format employed and suggest that clade C envelopes merit further evaluation for the elicitation of broadly neutralizing antibodies.

Original language	English
Pages (from-to)	529-538
Number of pages	10
Journal	Virology
Volume	375
Issue number	2
DOIs	https://doi.org/10.1016/j.virol.2008.02.022
State	Published - 5 Jun 2008
Externally published	Yes

Keywords

Cross-clade
HIV-1 neutralization
Primary isolate
Pseudovirus
TZM-bl reporter cells

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10.1016/j.virol.2008.02.022

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@article{b0242842e6a94d13972518b2acf6e374,

title = "Cross-clade neutralization patterns among HIV-1 strains from the six major clades of the pandemic evaluated and compared in two different models",

abstract = "A panel of paired primary virus isolates and envelope pseudoviruses from sixty strains representing six HIV-1 clades was tested for neutralization using pooled, clade-specific plasma in two prominently utilized neutralization platforms: a primary isolate assay using peripheral blood mononuclear cells (PBMC) and a pseudovirus assay using a reporter epithelial cell line. Using the PMBC assay, pairing of the antibody pool against homologous clade viruses generated the highest geometric mean neutralizing antibody titer in 4 out of 6 clades tested, and neutralization patterns showed numerous examples of reciprocal cross-recognition between antibody and viruses of specific clade pairs. In the pseudovirus assay, cross-clade neutralization was more limited, with fewer distinct cross-clade relationships evident. The clade C antibody pool was broadly cross-reactive, neutralizing the greatest number of viruses in both assays. These data highlight the importance of the neutralization assay format employed and suggest that clade C envelopes merit further evaluation for the elicitation of broadly neutralizing antibodies.",

keywords = "Cross-clade, HIV-1 neutralization, Primary isolate, Pseudovirus, TZM-bl reporter cells",

author = "Brown, {Bruce K.} and Lindsay Wieczorek and Eric Sanders-Buell and Borges, {Andrew Rosa} and Robb, {Merlin L.} and Birx, {Deborah L.} and Michael, {Nelson L.} and McCutchan, {Francine E.} and Polonis, {Victoria R.}",

note = "Funding Information: This work was supported in part by Cooperative Agreement no. DAMD17-93-V-3004, between the U.S. Army Medical Research and Material Command and the Henry M. Jackson Foundation for the Advancement of Military Medicine, working with the Division of AIDS, National Institute for Allergy and Infectious Diseases National Institutes of Health. The views expressed here are the private opinions of the authors and are not to be considered as official or reflecting the views of the U.S. Army of the U.S. Department of Defense. Use of trade names is for identification only and does not imply endorsement by the U.S. government.",

year = "2008",

month = jun,

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doi = "10.1016/j.virol.2008.02.022",

language = "English",

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pages = "529--538",

journal = "Virology",

issn = "0042-6822",

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T1 - Cross-clade neutralization patterns among HIV-1 strains from the six major clades of the pandemic evaluated and compared in two different models

AU - Brown, Bruce K.

AU - Wieczorek, Lindsay

AU - Sanders-Buell, Eric

AU - Borges, Andrew Rosa

AU - Robb, Merlin L.

AU - Birx, Deborah L.

AU - Michael, Nelson L.

AU - McCutchan, Francine E.

AU - Polonis, Victoria R.

N1 - Funding Information: This work was supported in part by Cooperative Agreement no. DAMD17-93-V-3004, between the U.S. Army Medical Research and Material Command and the Henry M. Jackson Foundation for the Advancement of Military Medicine, working with the Division of AIDS, National Institute for Allergy and Infectious Diseases National Institutes of Health. The views expressed here are the private opinions of the authors and are not to be considered as official or reflecting the views of the U.S. Army of the U.S. Department of Defense. Use of trade names is for identification only and does not imply endorsement by the U.S. government.

PY - 2008/6/5

Y1 - 2008/6/5

N2 - A panel of paired primary virus isolates and envelope pseudoviruses from sixty strains representing six HIV-1 clades was tested for neutralization using pooled, clade-specific plasma in two prominently utilized neutralization platforms: a primary isolate assay using peripheral blood mononuclear cells (PBMC) and a pseudovirus assay using a reporter epithelial cell line. Using the PMBC assay, pairing of the antibody pool against homologous clade viruses generated the highest geometric mean neutralizing antibody titer in 4 out of 6 clades tested, and neutralization patterns showed numerous examples of reciprocal cross-recognition between antibody and viruses of specific clade pairs. In the pseudovirus assay, cross-clade neutralization was more limited, with fewer distinct cross-clade relationships evident. The clade C antibody pool was broadly cross-reactive, neutralizing the greatest number of viruses in both assays. These data highlight the importance of the neutralization assay format employed and suggest that clade C envelopes merit further evaluation for the elicitation of broadly neutralizing antibodies.

AB - A panel of paired primary virus isolates and envelope pseudoviruses from sixty strains representing six HIV-1 clades was tested for neutralization using pooled, clade-specific plasma in two prominently utilized neutralization platforms: a primary isolate assay using peripheral blood mononuclear cells (PBMC) and a pseudovirus assay using a reporter epithelial cell line. Using the PMBC assay, pairing of the antibody pool against homologous clade viruses generated the highest geometric mean neutralizing antibody titer in 4 out of 6 clades tested, and neutralization patterns showed numerous examples of reciprocal cross-recognition between antibody and viruses of specific clade pairs. In the pseudovirus assay, cross-clade neutralization was more limited, with fewer distinct cross-clade relationships evident. The clade C antibody pool was broadly cross-reactive, neutralizing the greatest number of viruses in both assays. These data highlight the importance of the neutralization assay format employed and suggest that clade C envelopes merit further evaluation for the elicitation of broadly neutralizing antibodies.

KW - Cross-clade

KW - HIV-1 neutralization

KW - Primary isolate

KW - Pseudovirus

KW - TZM-bl reporter cells

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ER -

Cross-clade neutralization patterns among HIV-1 strains from the six major clades of the pandemic evaluated and compared in two different models

Abstract

Keywords

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