TY - JOUR
T1 - CSF proteome
T2 - A protein repository for potential biomarker identification
AU - Romeo, Martin J.
AU - Espina, Virginia
AU - Lowenthal, Mark
AU - Espina, Benjamin H.
AU - Petricoin, Emanuel F.
AU - Liotta, Lance A.
N1 - Funding Information:
Virginia Espina, MS National Institutes of Health, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bldg 10 Room 2A33, Bethesda, MD 20892, USA Tel.: +1 301 435 7763 Fax: +1 301 402 0043 [email protected] Mark Lowenthal, PhD National Institutes of Health, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bldg 10 Room 2A33, Bethesda, MD 20892, USA Tel.: +1 301 496 4873 Fax: +1 301 402 0043 [email protected] Benjamin H Espina National Institutes of Health, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bldg 10 Room 2A33, Bethesda, MD 20892, USA; & Our Lady of Good Counsel High School, Wheaton, MD, USA Tel.: +1 301 496 4873 Fax: +1 301 402 0043 [email protected] Emanuel F Petricoin III, PhD Food and Drug Administration, Office of Cellular and Gene Therapy, Center for Biologics Evaluation and Research, Bldg 29A Room 2D12, 8800 Rockville Pike, Bethesda, MD 20892, USA Tel.: +1 301 827 1797 Fax: +1 301 480 5005 [email protected] Lance A Liotta, MD PhD National Institutes of Health, Laboratory of Pathology, Center for Cancer Research, National Cancer Institute, 9000 Rockville Pike, Bldg 10 Room 2A33, Bethesda, MD 20892, USA Tel.: +1 301 496 2035 Fax: +1 301 480 0853 [email protected]
PY - 2005/2
Y1 - 2005/2
N2 - Proteomic analysis is not limited to the analysis of serum or tissues. Synovial, peritoneal, pericardial and cerebrospinal fluid represent unique proteomes for disease diagnosis and prognosis. In particular, cerebrospinal fluid serves as a rich source of putative biomarkers that are not solely limited to neurologic disorders. Peptides, proteolytic fragments and antibodies are capable of crossing the blood-brain barrier, thus providing a repository of pathologic information. Proteomic technologies such as immunoblotting, isoelectric focusing, 2D gel electrophoresis and mass spectrometry have proven useful for deciphering this unique proteome. Cerebrospinal fluid proteins are generally less abundant than their corresponding serum counterparts, necessitating the development and use of sensitive analytical techniques. This review highlights some of the promising areas of cerebrospinal fluid proteomic research and their clinical applications.
AB - Proteomic analysis is not limited to the analysis of serum or tissues. Synovial, peritoneal, pericardial and cerebrospinal fluid represent unique proteomes for disease diagnosis and prognosis. In particular, cerebrospinal fluid serves as a rich source of putative biomarkers that are not solely limited to neurologic disorders. Peptides, proteolytic fragments and antibodies are capable of crossing the blood-brain barrier, thus providing a repository of pathologic information. Proteomic technologies such as immunoblotting, isoelectric focusing, 2D gel electrophoresis and mass spectrometry have proven useful for deciphering this unique proteome. Cerebrospinal fluid proteins are generally less abundant than their corresponding serum counterparts, necessitating the development and use of sensitive analytical techniques. This review highlights some of the promising areas of cerebrospinal fluid proteomic research and their clinical applications.
KW - 2D gel electrophoresis
KW - Biomarker
KW - Central nervous system
KW - Cerebrospinal fluid
KW - ELISA
KW - Immunoblotting
KW - Isoelectric focusing
KW - MALDI
KW - Mass spectrometry
KW - Neurodegenerative
KW - Protein
KW - Proteomics
UR - http://www.scopus.com/inward/record.url?scp=14344256012&partnerID=8YFLogxK
U2 - 10.1586/14789450.2.1.57
DO - 10.1586/14789450.2.1.57
M3 - Review article
C2 - 15966853
AN - SCOPUS:14344256012
SN - 1478-9450
VL - 2
SP - 57
EP - 70
JO - Expert Review of Proteomics
JF - Expert Review of Proteomics
IS - 1
ER -