DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells

Boonrat Tassaneetrithep; Timothy H. Burgess; Angela Granelli-Piperno; Christine Trumpfheller; Jennifer Finke; Wellington Sun; Michael A. Eller; Kovit Pattanapanyasat; Suttipant Sarasombath; Deborah L. Birx; Ralph M. Steinman; Sarah Schlesinger; Mary A. Marovich

doi:10.1084/jem.20021840

DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells

Boonrat Tassaneetrithep, Timothy H. Burgess, Angela Granelli-Piperno, Christine Trumpfheller, Jennifer Finke, Wellington Sun, Michael A. Eller, Kovit Pattanapanyasat, Suttipant Sarasombath, Deborah L. Birx, Ralph M. Steinman, Sarah Schlesinger, Mary A. Marovich^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

735 Scopus citations

Abstract

Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.

Original language	English
Pages (from-to)	823-829
Number of pages	7
Journal	Journal of Experimental Medicine
Volume	197
Issue number	7
DOIs	https://doi.org/10.1084/jem.20021840
State	Published - Apr 2003
Externally published	Yes

Keywords

Antigen-presenting cells
Flavivirus
Lectin
Receptor
Virus receptor

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10.1084/jem.20021840

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Tassaneetrithep, B., Burgess, T. H., Granelli-Piperno, A., Trumpfheller, C., Finke, J., Sun, W., Eller, M. A., Pattanapanyasat, K., Sarasombath, S., Birx, D. L., Steinman, R. M., Schlesinger, S., & Marovich, M. A. (2003). DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells. Journal of Experimental Medicine, 197(7), 823-829. https://doi.org/10.1084/jem.20021840

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title = "DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells",

abstract = "Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.",

keywords = "Antigen-presenting cells, Flavivirus, Lectin, Receptor, Virus receptor",

author = "Boonrat Tassaneetrithep and Burgess, {Timothy H.} and Angela Granelli-Piperno and Christine Trumpfheller and Jennifer Finke and Wellington Sun and Eller, {Michael A.} and Kovit Pattanapanyasat and Suttipant Sarasombath and Birx, {Deborah L.} and Steinman, {Ralph M.} and Sarah Schlesinger and Marovich, {Mary A.}",

year = "2003",

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doi = "10.1084/jem.20021840",

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Tassaneetrithep, B, Burgess, TH, Granelli-Piperno, A, Trumpfheller, C, Finke, J, Sun, W, Eller, MA, Pattanapanyasat, K, Sarasombath, S, Birx, DL, Steinman, RM, Schlesinger, S & Marovich, MA 2003, 'DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells', Journal of Experimental Medicine, vol. 197, no. 7, pp. 823-829. https://doi.org/10.1084/jem.20021840

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AU - Tassaneetrithep, Boonrat

AU - Burgess, Timothy H.

AU - Granelli-Piperno, Angela

AU - Trumpfheller, Christine

AU - Finke, Jennifer

AU - Sun, Wellington

AU - Eller, Michael A.

AU - Pattanapanyasat, Kovit

AU - Sarasombath, Suttipant

AU - Birx, Deborah L.

AU - Steinman, Ralph M.

AU - Schlesinger, Sarah

AU - Marovich, Mary A.

PY - 2003/4

Y1 - 2003/4

N2 - Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.

AB - Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.

KW - Antigen-presenting cells

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KW - Lectin

KW - Receptor

KW - Virus receptor

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DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells

Abstract

Keywords

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