DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells

Boonrat Tassaneetrithep, Timothy H. Burgess, Angela Granelli-Piperno, Christine Trumpfheller, Jennifer Finke, Wellington Sun, Michael A. Eller, Kovit Pattanapanyasat, Suttipant Sarasombath, Deborah L. Birx, Ralph M. Steinman, Sarah Schlesinger, Mary A. Marovich*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

723 Scopus citations

Abstract

Dengue virus is a single-stranded, enveloped RNA virus that productively infects human dendritic cells (DCs) primarily at the immature stage of their differentiation. We now find that all four serotypes of dengue use DC-SIGN (CD209), a C-type lectin, to infect dendritic cells. THP-1 cells become susceptible to dengue infection after transfection of DC-specific ICAM-3 grabbing nonintegrin (DC-SIGN), or its homologue L-SIGN, whereas the infection of dendritic cells is blocked by anti-DC-SIGN antibodies and not by antibodies to other molecules on these cells. Viruses produced by dendritic cells are infectious for DC-SIGN- and L-SIGN-bearing THP-1 cells and other permissive cell lines. Therefore, DC-SIGN may be considered as a new target for designing therapies that block dengue infection.

Original languageEnglish
Pages (from-to)823-829
Number of pages7
JournalJournal of Experimental Medicine
Volume197
Issue number7
DOIs
StatePublished - Apr 2003
Externally publishedYes

Keywords

  • Antigen-presenting cells
  • Flavivirus
  • Lectin
  • Receptor
  • Virus receptor

Fingerprint

Dive into the research topics of 'DC-SIGN (CD209) mediates dengue virus infection of human dendritic cells'. Together they form a unique fingerprint.

Cite this