Decellularization and cell seeding of whole liver biologic scaffolds composed of extracellular matrix

Denver M. Faulk, Justin D. Wildemann, Stephen F. Badylak*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

84 Scopus citations

Abstract

The definitive treatment for patients with end-stage liver disease is orthotropic transplantation. However, this option is limited by the disparity between the number of patients needing transplantation and the number of available livers. This issue is becoming more severe as the population ages and as the number of new cases of end-stage liver failure increases. Patients fortunate enough to receive a transplant are required to receive immunosuppressive therapy and must live with the associated morbidity. Whole organ engineering of the liver may offer a solution to this liver donor shortfall. It has been shown that perfusion decellularization of a whole allogeneic or xenogeneic liver generates a three-dimensional ECM scaffold with intact macro and micro architecture of the native liver. A decellularized liver provides an ideal transplantable scaffold with all the necessary ultrastructure and signaling cues for cell attachment, differentiation, vascularization, and function. In this review, an overview of complementary strategies for creating functional liver grafts suitable for transplantation is provided. Early milestones have been met by combining stem and progenitor cells with increasingly complex scaffold materials and culture conditions.

Original languageEnglish
Pages (from-to)69-80
Number of pages12
JournalJournal of Clinical and Experimental Hepatology
Volume5
Issue number1
DOIs
StatePublished - 1 Mar 2015
Externally publishedYes

Keywords

  • Biologic scaffold
  • Decellularization
  • Extracellular matrix
  • Liver tissue engineering
  • Organ engineering

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