Decellularization of tissues and organs

Thomas W. Gilbert, Tiffany L. Sellaro, Stephen F. Badylak*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

1911 Scopus citations

Abstract

Decellularized tissues and organs have been successfully used in a variety of tissue engineering/regenerative medicine applications, and the decellularization methods used vary as widely as the tissues and organs of interest. The efficiency of cell removal from a tissue is dependent on the origin of the tissue and the specific physical, chemical, and enzymatic methods that are used. Each of these treatments affect the biochemical composition, tissue ultrastructure, and mechanical behavior of the remaining extracellular matrix (ECM) scaffold, which in turn, affect the host response to the material. Herein, the most commonly used decellularization methods are described, and consideration give to the effects of these methods upon the biologic scaffold material.

Original languageEnglish
Pages (from-to)3675-3683
Number of pages9
JournalBiomaterials
Volume27
Issue number19
DOIs
StatePublished - Jul 2006
Externally publishedYes

Keywords

  • Decellularization
  • Extracellular matrix
  • Scaffolds
  • Tissue engineering

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