Defining genetic diversity of rhesus macaque Fcγ receptors with long-read RNA sequencing

Haleigh E. Conley, Max M. He, David Easterhoff, Hélène Fradin Kirshner, Sarah L. Cocklin, Jacob Meyer, Taylor Hoxie, Madison Berry, Todd Bradley, William D. Tolbert, Marzena Pazgier, Georgia D. Tomaras, Joern E. Schmitz, Michael Anthony Moody, Kevin Wiehe*, Justin Pollara*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Fcγ receptors (FcγRs) are membrane-bound glycoproteins that bind to the fragment crystallizable (Fc) constant regions of IgG antibodies. Interactions between IgG immune complexes and FcγRs can initiate signal transduction that mediates important components of the immune response including activation of immune cells for clearance of opsonized pathogens or infected host cells. In humans, many studies have identified associations between FcγR gene polymorphisms and risk of infection, or progression of disease, suggesting a gene-level impact on FcγR-dependent immune responses. Rhesus macaques are an important translational model for most human health interventions, yet little is known about the breadth of rhesus macaque FcγR genetic diversity. This lack of knowledge prevents evaluation of the impact of FcγR polymorphisms on outcomes of preclinical studies performed in rhesus macaques. In this study we used long-read RNA sequencing to define the genetic diversity of FcγRs in 206 Indian-origin Rhesus macaques, Macaca mulatta. We describe the frequency of single nucleotide polymorphisms, insertions, deletions, frame-shift mutations, and isoforms. We also index the identified diversity using predicted and known rhesus macaque FcγR and Fc-FcγR structures. Future studies that define the functional significance of this genetic diversity will facilitate a better understanding of the correlation between human and macaque FcγR biology that is needed for effective translation of studies with antibody-mediated outcomes performed in rhesus macaques.

Original languageEnglish
Article number1306292
JournalFrontiers in Immunology
StatePublished - 2023
Externally publishedYes


  • Fc receptor
  • FcγR SNPs
  • FcγR structures
  • Long-read RNA sequencing
  • genetic diversity
  • rhesus macaques


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