TY - JOUR
T1 - Dengue virus type 1 DNA vaccine induces protective immune responses in rhesus macaques
AU - Raviprakash, Kanakatte
AU - Porter, Kevin R.
AU - Kochel, Tadeuscz J.
AU - Ewing, Daniel
AU - Simmons, Monica
AU - Phillips, Irving
AU - Murphy, Gerald S.
AU - Weiss, Walter R.
AU - Hayes, Curtis G.
PY - 2000
Y1 - 2000
N2 - A candidate DNA vaccine expressing dengue virus type 1 pre-membrane and envelope proteins was used to immunize rhesus macaques. Monkeys were immunized intramuscularly (i.m.) or intradermally (i.d.) by three or four 1 mg doses of vaccine, respectively. Monkeys that were inoculated i.m. seroconverted more quickly and had higher antibody levels than those that were inoculated i.d. The sera exhibited virus-neutralizing activity, which declined over time. Four of the eight i.m.-inoculated monkeys were protected completely from developing viraemia when challenged 4 months after the last dose with homologous dengue virus. The other four monkeys had reduced viraemia compared with the control immunized monkeys. The i.d.-inoculated monkeys showed no reduction in viraemia when challenged with the virus. All vaccinated monkeys showed an anamnestic antibody response, indicating that they had established immunological memory. Vaccine-induced antibody had an avidity index similar to that of antibody induced by virus infection; however, no clear correlation was apparent between antibody avidity and virus neutralization titres.
AB - A candidate DNA vaccine expressing dengue virus type 1 pre-membrane and envelope proteins was used to immunize rhesus macaques. Monkeys were immunized intramuscularly (i.m.) or intradermally (i.d.) by three or four 1 mg doses of vaccine, respectively. Monkeys that were inoculated i.m. seroconverted more quickly and had higher antibody levels than those that were inoculated i.d. The sera exhibited virus-neutralizing activity, which declined over time. Four of the eight i.m.-inoculated monkeys were protected completely from developing viraemia when challenged 4 months after the last dose with homologous dengue virus. The other four monkeys had reduced viraemia compared with the control immunized monkeys. The i.d.-inoculated monkeys showed no reduction in viraemia when challenged with the virus. All vaccinated monkeys showed an anamnestic antibody response, indicating that they had established immunological memory. Vaccine-induced antibody had an avidity index similar to that of antibody induced by virus infection; however, no clear correlation was apparent between antibody avidity and virus neutralization titres.
UR - http://www.scopus.com/inward/record.url?scp=0033920796&partnerID=8YFLogxK
U2 - 10.1099/0022-1317-81-7-1659
DO - 10.1099/0022-1317-81-7-1659
M3 - Article
C2 - 10859370
AN - SCOPUS:0033920796
SN - 0022-1317
VL - 81
SP - 1659
EP - 1667
JO - Journal of General Virology
JF - Journal of General Virology
IS - 7
ER -