@article{4e86713fa5294254ab580570bd45bc03,
title = "Design and evaluation of multi-gene, multi-clade HIV-1 MVA vaccines",
abstract = "Recombinant modified vaccinia virus Ankara (rMVA) expressing HIV-1 genes are promising vaccine candidates. Toward the goal of conducting clinical trials with one or a cocktail of recombinant viruses, four rMVAs expressing env and gag-pol genes from primary HIV-1 isolates representing predominant subtypes from Kenya, Tanzania, Uganda, and Thailand (A, C, D, and CRF01_AE, respectively) were constructed. Efficient expression, processing, and function of Env and Gag were demonstrated. All inserted genes were shown to be genetically stable after repeated passage in cell culture. Strong HIV-specific cellular and humoral immune responses were elicited in mice immunized with each individual vaccine candidate. The MVA/CMDR vaccine candidate expressing CRF01_AE genes has elicited HIV-specific T-cell responses in two independent Phase I clinical trials. Further testing of the other rMVA is warranted.",
keywords = "HIV vaccine, Immune response, MVA",
author = "Earl, {Patricia L.} and Catherine Cotter and Bernard Moss and Thomas VanCott and Jeffrey Currier and Eller, {Leigh Anne} and Francine McCutchan and Birx, {Deborah L.} and Michael, {Nelson L.} and Marovich, {Mary A.} and Merlin Robb and Cox, {Josephine H.}",
note = "Funding Information: This work was supported in part by the Intramural Research Program, National Institute of Allergy and Infectious Diseases, National Institutes of Health and by the U.S. Army Medical Research and Materiel Command and its Cooperative Agreement (DAMD17-98-2-7007) with the Henry M. Jackson Foundation for the Advancement of Military Medicine. The opinions or assertions contained herein are the private views of the author, and are not to be construed as official, or as reflecting true views of the Department of the Army or the Department of Defense. Research was conducted in compliance with the Animal Welfare Act and other federal statutes and regulations relating to animals and experiments involving animals and adheres to principles stated in the Guide for the Care and Use of Laboratory Animals , NRC Publication, 1996 edition. ",
year = "2009",
month = sep,
day = "25",
doi = "10.1016/j.vaccine.2009.07.039",
language = "English",
volume = "27",
pages = "5885--5895",
journal = "Vaccine",
issn = "0264-410X",
number = "42",
}