Detection of conformation bias between the complementary strands of deoxynucleotide repeat (TG/AC)(n)

Albert Dobi; Mark A. Mahan; Denes V. Agoston

doi:10.1002/elps.1150180104

Detection of conformation bias between the complementary strands of deoxynucleotide repeat (TG/AC)(n)

Albert Dobi, Mark A. Mahan, Denes V. Agoston^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differences between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences between complementary strands,we challenged the complementary strands to different 'pore size' of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in migration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy-(TG/AC)(n).

Original language	English
Pages (from-to)	12-16
Number of pages	5
Journal	Electrophoresis
Volume	18
Issue number	1
DOIs	https://doi.org/10.1002/elps.1150180104
State	Published - Jan 1997
Externally published	Yes

Keywords

DNA
DNA-repeat
Nucleic acd conformation
Polyacrylamide gel electrophoresis

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title = "Detection of conformation bias between the complementary strands of deoxynucleotide repeat (TG/AC)(n)",

abstract = "Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differences between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences between complementary strands,we challenged the complementary strands to different 'pore size' of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in migration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy-(TG/AC)(n).",

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AU - Dobi, Albert

AU - Mahan, Mark A.

AU - Agoston, Denes V.

PY - 1997/1

Y1 - 1997/1

N2 - Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differences between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences between complementary strands,we challenged the complementary strands to different 'pore size' of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in migration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy-(TG/AC)(n).

AB - Antiparallel complementary strands of DNA contribute symmetrically to the helical structure and its stability; rough conformational differences between complementary strands would interfere with this symmetric contribution and the stability. To detect conformational differences between complementary strands,we challenged the complementary strands to different 'pore size' of acrylamide gels and measured differences in migration during electrophoretic mobility analysis. Changes in migration differences revealed a significant structural bias between the complementary strands of the dinucleotide repeat deoxy-(TG/AC)(n).

KW - DNA

KW - DNA-repeat

KW - Nucleic acd conformation

KW - Polyacrylamide gel electrophoresis

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U2 - 10.1002/elps.1150180104

DO - 10.1002/elps.1150180104

M3 - Article

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VL - 18

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EP - 16

JO - Electrophoresis

JF - Electrophoresis

IS - 1

ER -

Detection of conformation bias between the complementary strands of deoxynucleotide repeat (TG/AC)(n)

Abstract

Keywords

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