Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Tahaniyat Lalani; Michele D. Tisdale; Jason D. Maguire; Chansuda Wongsrichanalai; Mark S. Riddle; David R. Tribble

doi:10.1016/j.diagmicrobio.2015.05.011

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Tahaniyat Lalani^*, Michele D. Tisdale, Jason D. Maguire, Chansuda Wongsrichanalai, Mark S. Riddle, David R. Tribble

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman™ FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 10² to 10⁵CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.

Original language	English
Pages (from-to)	18-20
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	83
Issue number	1
DOIs	https://doi.org/10.1016/j.diagmicrobio.2015.05.011
State	Published - 1 Sep 2015

Keywords

PCR
Stool card
Travelers' diarrhea

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10.1016/j.diagmicrobio.2015.05.011

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Lalani, T., Tisdale, M. D., Maguire, J. D., Wongsrichanalai, C., Riddle, M. S., & Tribble, D. R. (2015). Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards. Diagnostic Microbiology and Infectious Disease, 83(1), 18-20. https://doi.org/10.1016/j.diagmicrobio.2015.05.011

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title = "Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards",

abstract = "We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman{\texttrademark} FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 102 to 105CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.",

keywords = "PCR, Stool card, Travelers' diarrhea",

author = "Tahaniyat Lalani and Tisdale, {Michele D.} and Maguire, {Jason D.} and Chansuda Wongsrichanalai and Riddle, {Mark S.} and Tribble, {David R.}",

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doi = "10.1016/j.diagmicrobio.2015.05.011",

language = "English",

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Lalani, T, Tisdale, MD, Maguire, JD, Wongsrichanalai, C, Riddle, MS & Tribble, DR 2015, 'Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards', Diagnostic Microbiology and Infectious Disease, vol. 83, no. 1, pp. 18-20. https://doi.org/10.1016/j.diagmicrobio.2015.05.011

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards. / Lalani, Tahaniyat; Tisdale, Michele D.; Maguire, Jason D. et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 83, No. 1, 01.09.2015, p. 18-20.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

AU - Lalani, Tahaniyat

AU - Tisdale, Michele D.

AU - Maguire, Jason D.

AU - Wongsrichanalai, Chansuda

AU - Riddle, Mark S.

AU - Tribble, David R.

PY - 2015/9/1

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N2 - We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman™ FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 102 to 105CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.

AB - We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman™ FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 102 to 105CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.

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IS - 1

ER -

Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Abstract

Keywords

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