Detection of enteropathogens associated with travelers' diarrhea using a multiplex Luminex-based assay performed on stool samples smeared on Whatman FTA Elute cards

Tahaniyat Lalani*, Michele D. Tisdale, Jason D. Maguire, Chansuda Wongsrichanalai, Mark S. Riddle, David R. Tribble

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

We evaluated the limits of detection (LoD) for an 11-plex PCR-Luminex assay performed on Whatman™ FTA Elute cards smeared with stool containing pathogens associated with travelers' diarrhea. LoDs ranged from 102 to 105CFU, PFU, or cysts/g for most pathogens except Cryptosporidium. Campylobacter and norovirus LoDs increased with prolonged storage of cards.

Original languageEnglish
Pages (from-to)18-20
Number of pages3
JournalDiagnostic Microbiology and Infectious Disease
Volume83
Issue number1
DOIs
StatePublished - 1 Sep 2015

Keywords

  • PCR
  • Stool card
  • Travelers' diarrhea

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