Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3

Kristi Y. Lee; Erica L. Beatson; Martina A. Knechel; Elijah R. Sommer; Giulia C. Napoli; Emily N. Risdon; Andres F. Leon; Roger D. Depaz; Jonathan D. Strope; Douglas K. Price; Cindy H. Chau; William D. Figg

doi:10.7150/jca.90836

Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3

Kristi Y. Lee, Erica L. Beatson, Martina A. Knechel, Elijah R. Sommer, Giulia C. Napoli, Emily N. Risdon, Andres F. Leon, Roger D. Depaz, Jonathan D. Strope, Douglas K. Price, Cindy H. Chau, William D. Figg^*

^*Corresponding author for this work

Surgery

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Abstract

Extracellular vesicles (EVs) provide a minimally invasive liquid biopsy source of tumor-specific markers for patients who have already undergone prostatectomies. Our laboratory has previously demonstrated enrichment of the cancer-type solute carrier organic anion transporter family 1B3 (ct-SLCO1B3) and the ATP Binding Cassette Subfamily Member C (ABCC3) in castration-resistant cell lines (CRPC). However, their expression in EVs has yet to be explored. Our study demonstrated that ct-SLCO1B3 and ABCC3 are highly detectable in CRPC cell line-derived EVs. We also showed that ct-SLCO1B3 and ABCC3 were detectable in a CRPC xenograft mouse model, both intratumorally and in plasma-derived EVs. Our results provide evidence for EV-contained ct-SLCO1B3 and ABCC3 as novel, EV-based tumor markers for prostate cancer progression.

Original language	English
Pages (from-to)	615-622
Number of pages	8
Journal	Journal of Cancer
Volume	15
Issue number	3
DOIs	https://doi.org/10.7150/jca.90836
State	Published - 2024

Keywords

ABCC3
Organic anion transporter family 1B3
SLCO1B3
cancer biomarker
extracellular vesicle
prostate cancer

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10.7150/jca.90836

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Lee, K. Y., Beatson, E. L., Knechel, M. A., Sommer, E. R., Napoli, G. C., Risdon, E. N., Leon, A. F., Depaz, R. D., Strope, J. D., Price, D. K., Chau, C. H., & Figg, W. D. (2024). Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3. Journal of Cancer, 15(3), 615-622. https://doi.org/10.7150/jca.90836

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title = "Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3",

abstract = "Extracellular vesicles (EVs) provide a minimally invasive liquid biopsy source of tumor-specific markers for patients who have already undergone prostatectomies. Our laboratory has previously demonstrated enrichment of the cancer-type solute carrier organic anion transporter family 1B3 (ct-SLCO1B3) and the ATP Binding Cassette Subfamily Member C (ABCC3) in castration-resistant cell lines (CRPC). However, their expression in EVs has yet to be explored. Our study demonstrated that ct-SLCO1B3 and ABCC3 are highly detectable in CRPC cell line-derived EVs. We also showed that ct-SLCO1B3 and ABCC3 were detectable in a CRPC xenograft mouse model, both intratumorally and in plasma-derived EVs. Our results provide evidence for EV-contained ct-SLCO1B3 and ABCC3 as novel, EV-based tumor markers for prostate cancer progression.",

keywords = "ABCC3, Organic anion transporter family 1B3, SLCO1B3, cancer biomarker, extracellular vesicle, prostate cancer",

author = "Lee, {Kristi Y.} and Beatson, {Erica L.} and Knechel, {Martina A.} and Sommer, {Elijah R.} and Napoli, {Giulia C.} and Risdon, {Emily N.} and Leon, {Andres F.} and Depaz, {Roger D.} and Strope, {Jonathan D.} and Price, {Douglas K.} and Chau, {Cindy H.} and Figg, {William D.}",

year = "2024",

doi = "10.7150/jca.90836",

language = "English",

volume = "15",

pages = "615--622",

journal = "Journal of Cancer",

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T1 - Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers

T2 - Role of Cancer-type SLCO1B3 and ABCC3

AU - Lee, Kristi Y.

AU - Beatson, Erica L.

AU - Knechel, Martina A.

AU - Sommer, Elijah R.

AU - Napoli, Giulia C.

AU - Risdon, Emily N.

AU - Leon, Andres F.

AU - Depaz, Roger D.

AU - Strope, Jonathan D.

AU - Price, Douglas K.

AU - Chau, Cindy H.

AU - Figg, William D.

PY - 2024

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N2 - Extracellular vesicles (EVs) provide a minimally invasive liquid biopsy source of tumor-specific markers for patients who have already undergone prostatectomies. Our laboratory has previously demonstrated enrichment of the cancer-type solute carrier organic anion transporter family 1B3 (ct-SLCO1B3) and the ATP Binding Cassette Subfamily Member C (ABCC3) in castration-resistant cell lines (CRPC). However, their expression in EVs has yet to be explored. Our study demonstrated that ct-SLCO1B3 and ABCC3 are highly detectable in CRPC cell line-derived EVs. We also showed that ct-SLCO1B3 and ABCC3 were detectable in a CRPC xenograft mouse model, both intratumorally and in plasma-derived EVs. Our results provide evidence for EV-contained ct-SLCO1B3 and ABCC3 as novel, EV-based tumor markers for prostate cancer progression.

AB - Extracellular vesicles (EVs) provide a minimally invasive liquid biopsy source of tumor-specific markers for patients who have already undergone prostatectomies. Our laboratory has previously demonstrated enrichment of the cancer-type solute carrier organic anion transporter family 1B3 (ct-SLCO1B3) and the ATP Binding Cassette Subfamily Member C (ABCC3) in castration-resistant cell lines (CRPC). However, their expression in EVs has yet to be explored. Our study demonstrated that ct-SLCO1B3 and ABCC3 are highly detectable in CRPC cell line-derived EVs. We also showed that ct-SLCO1B3 and ABCC3 were detectable in a CRPC xenograft mouse model, both intratumorally and in plasma-derived EVs. Our results provide evidence for EV-contained ct-SLCO1B3 and ABCC3 as novel, EV-based tumor markers for prostate cancer progression.

KW - ABCC3

KW - Organic anion transporter family 1B3

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KW - cancer biomarker

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JF - Journal of Cancer

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ER -

Detection of Extracellular Vesicle-Derived RNA as Potential Prostate Cancer Biomarkers: Role of Cancer-type SLCO1B3 and ABCC3

Abstract

Keywords

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