Detection of Francisella tularensis in blood by polymerase chain reaction

G. W. Long*, J. J. Oprandy, R. B. Narayanan, A. H. Fortier, K. R. Porter, C. A. Nacy

*Corresponding author for this work

Research output: Contribution to journalComment/debate

82 Scopus citations

Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

Original languageEnglish
Pages (from-to)152-154
Number of pages3
JournalJournal of Clinical Microbiology
Volume31
Issue number1
DOIs
StatePublished - 1993
Externally publishedYes

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