Abstract
We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.
Original language | English |
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Pages (from-to) | 152-154 |
Number of pages | 3 |
Journal | Journal of Clinical Microbiology |
Volume | 31 |
Issue number | 1 |
DOIs |
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State | Published - 1993 |
Externally published | Yes |