Detection of Francisella tularensis in blood by polymerase chain reaction

G. W. Long; J. J. Oprandy; R. B. Narayanan; A. H. Fortier; K. R. Porter; C. A. Nacy

doi:10.1128/jcm.31.1.152-154.1993

Detection of Francisella tularensis in blood by polymerase chain reaction

G. W. Long^*
, J. J. Oprandy
, R. B. Narayanan
, A. H. Fortier
, K. R. Porter
, C. A. Nacy

^*Corresponding author for this work

Research output: Contribution to journal › Comment/debate

87 Scopus citations

Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

Original language	English
Pages (from-to)	152-154
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1128/jcm.31.1.152-154.1993
State	Published - 1993
Externally published	Yes

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10.1128/jcm.31.1.152-154.1993

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title = "Detection of Francisella tularensis in blood by polymerase chain reaction",

abstract = "We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.",

author = "Long, \{G. W.\} and Oprandy, \{J. J.\} and Narayanan, \{R. B.\} and Fortier, \{A. H.\} and Porter, \{K. R.\} and Nacy, \{C. A.\}",

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doi = "10.1128/jcm.31.1.152-154.1993",

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AU - Long, G. W.

AU - Oprandy, J. J.

AU - Narayanan, R. B.

AU - Fortier, A. H.

AU - Porter, K. R.

AU - Nacy, C. A.

PY - 1993

Y1 - 1993

N2 - We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

AB - We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

UR - https://www.scopus.com/pages/publications/0027514532

U2 - 10.1128/jcm.31.1.152-154.1993

DO - 10.1128/jcm.31.1.152-154.1993

M3 - Comment/debate

C2 - 8417022

AN - SCOPUS:0027514532

SN - 0095-1137

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SP - 152

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JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 1

ER -

Detection of Francisella tularensis in blood by polymerase chain reaction

Abstract

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