Detection of Francisella tularensis in blood by polymerase chain reaction

G. W. Long; J. J. Oprandy; R. B. Narayanan; A. H. Fortier; K. R. Porter; C. A. Nacy

doi:10.1128/jcm.31.1.152-154.1993

Detection of Francisella tularensis in blood by polymerase chain reaction

G. W. Long^*, J. J. Oprandy, R. B. Narayanan, A. H. Fortier, K. R. Porter, C. A. Nacy

^*Corresponding author for this work

Research output: Contribution to journal › Comment/debate

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Abstract

We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

Original language	English
Pages (from-to)	152-154
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	31
Issue number	1
DOIs	https://doi.org/10.1128/jcm.31.1.152-154.1993
State	Published - 1993
Externally published	Yes

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10.1128/jcm.31.1.152-154.1993

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title = "Detection of Francisella tularensis in blood by polymerase chain reaction",

abstract = "We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.",

author = "Long, {G. W.} and Oprandy, {J. J.} and Narayanan, {R. B.} and Fortier, {A. H.} and Porter, {K. R.} and Nacy, {C. A.}",

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AU - Long, G. W.

AU - Oprandy, J. J.

AU - Narayanan, R. B.

AU - Fortier, A. H.

AU - Porter, K. R.

AU - Nacy, C. A.

PY - 1993

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AB - We developed a polymerase chain reaction-based assay for Francisella tularensis which we evaluated by using spiked blood samples and experimentally infected mice. The assay detected both type A and type B F. tularensis at levels equivalent to one CFU/μl of spiked blood. Results from polymerase chain reaction-based assay of limiting dilutions of blood from mice infected with the live vaccine strain agreed closely with results from blood culture.

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DO - 10.1128/jcm.31.1.152-154.1993

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AN - SCOPUS:0027514532

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ER -

Detection of Francisella tularensis in blood by polymerase chain reaction

Abstract

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