TY - JOUR
T1 - Detection of serum microRNAs from department of defense serum repository
AU - Woeller, Collynn F.
AU - Thatcher, Thomas H.
AU - Van Twisk, Daniel
AU - Pollock, Stephen J.
AU - Croasdell, Amanda
AU - Kim, Nina
AU - Hopke, Philip K.
AU - Xia, Xiaoyan
AU - Thakar, Juilee
AU - Mallon, Timothy M.
AU - Utell, Mark J.
AU - Phipps, Richard P.
N1 - Publisher Copyright:
© 2016 American College of Occupational and Environmental Medicine.
PY - 2016/8/1
Y1 - 2016/8/1
N2 - Objective: The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs). Methods: MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy. Results: We detected miRNAs, cytokines, IgE, and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine suggesting tobacco exposure. Significant associations between serum cotinine, cytokine, IgE, PAHs, and miRNA were discovered. Conclusion: We successfully quantified over 200 potential biomarkers of occupational exposure from DoDSR samples. The stored serum samples were not affected by hemolysis and represent a powerful tool for biomarker discovery and analysis in retrospective studies.
AB - Objective: The aim of this study was to investigate whether serum samples from the Department of Defense Serum Repository (DoDSR) are of sufficient quality to detect microRNAs (miRNAs), cytokines, immunoglobulin E (IgE), and polycyclic aromatic hydrocarbons (PAHs). Methods: MiRNAs were isolated and quantified by polymerase chain reaction (PCR) array. Cytokines and chemokines related to inflammation were measured using multiplex immunoassays. Cotinine and IgE were detected by enzyme-linked immunoassay (ELISA) and PAHs were detected by Liquid Chromatography/Mass Spectroscopy. Results: We detected miRNAs, cytokines, IgE, and PAHs with high sensitivity. Eleven of 30 samples tested positive for cotinine suggesting tobacco exposure. Significant associations between serum cotinine, cytokine, IgE, PAHs, and miRNA were discovered. Conclusion: We successfully quantified over 200 potential biomarkers of occupational exposure from DoDSR samples. The stored serum samples were not affected by hemolysis and represent a powerful tool for biomarker discovery and analysis in retrospective studies.
UR - http://www.scopus.com/inward/record.url?scp=84983350360&partnerID=8YFLogxK
U2 - 10.1097/JOM.0000000000000742
DO - 10.1097/JOM.0000000000000742
M3 - Article
C2 - 27501106
AN - SCOPUS:84983350360
SN - 1076-2752
VL - 58
SP - S62-S71
JO - Journal of Occupational and Environmental Medicine
JF - Journal of Occupational and Environmental Medicine
IS - 8S
ER -