Detection of Sickle cell hemoglobin in Haiti by genotyping and hemoglobin solubility tests

Tamar E. Carter*, Michael Von Fricken, Jean R. Romain, Gladys Memnon, Yves St. Victor, Laura Schick, Bernard A. Okech, Connie J. Mulligan

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


Sickle cell disease is a growing global health concern because infants born with the disorder in developing countries are now surviving longer with little access to diagnostic and management options. In Haiti, the current state of sickle cell disease/trait in the population is unclear. To inform future screening efforts in Haiti, we assayed sickle hemoglobin mutations using traditional hemoglobin solubility tests (HST) and add-on techniques, which incorporated spectrophotometry and insoluble hemoglobin separation. We also generated genotype data as a metric for HST performance. We found 19 of 202 individuals screened with HST were positive for sickle hemoglobin, five of whom did not carry the HbS allele. We show that spectrophotometry and insoluble hemoglobin separation add-on techniques could resolve false positives associated with the traditional HST approach, with some limitations. We also discuss the incorporation of insoluble hemoglobin separation observation with HST in suboptimal screening settings like Haiti.

Original languageEnglish
Pages (from-to)406-411
Number of pages6
JournalAmerican Journal of Tropical Medicine and Hygiene
Issue number2
StatePublished - Aug 2014
Externally publishedYes


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