TY - JOUR
T1 - Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry
AU - Hwang, Kyunghwa
AU - Acharya, Milin R.
AU - Sausville, Edward A.
AU - Zhai, Suoping
AU - Woo, Eunhee W.
AU - Venitz, Jürgen
AU - Figg, William D.
AU - Sparreboom, Alex
PY - 2004/5/25
Y1 - 2004/5/25
N2 - A rapid method was developed for the quantitative determination of the novel histone deacetylase inhibitor, MS-275, in human plasma. Calibration curves were constructed in the range of 1-100ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a one-step protein precipitation with acetonitrile of 0.1ml samples. The analysis was performed on a column (75mm ×4.6mm i.d.) packed with 3.5μm Phenyl-SB material, using methanol - 10mM ammonium formate (55:45 (v/v)) as the mobile phase. The column effluent was monitored by mass spectrometry with positive electrospray ionization. The values for precision and accuracy were always ≤5.58 and <11.4% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of MS-275 in a cancer patient.
AB - A rapid method was developed for the quantitative determination of the novel histone deacetylase inhibitor, MS-275, in human plasma. Calibration curves were constructed in the range of 1-100ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a one-step protein precipitation with acetonitrile of 0.1ml samples. The analysis was performed on a column (75mm ×4.6mm i.d.) packed with 3.5μm Phenyl-SB material, using methanol - 10mM ammonium formate (55:45 (v/v)) as the mobile phase. The column effluent was monitored by mass spectrometry with positive electrospray ionization. The values for precision and accuracy were always ≤5.58 and <11.4% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of MS-275 in a cancer patient.
KW - Histone deacetylase inhibitor
KW - MS-275
UR - http://www.scopus.com/inward/record.url?scp=1842737512&partnerID=8YFLogxK
U2 - 10.1016/j.jchromb.2004.01.023
DO - 10.1016/j.jchromb.2004.01.023
M3 - Article
C2 - 15081922
AN - SCOPUS:1842737512
SN - 1570-0232
VL - 804
SP - 289
EP - 294
JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
IS - 2
ER -