Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry

Kyunghwa Hwang; Milin R. Acharya; Edward A. Sausville; Suoping Zhai; Eunhee W. Woo; Jürgen Venitz; William D. Figg; Alex Sparreboom

doi:10.1016/j.jchromb.2004.01.023

Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry

Kyunghwa Hwang, Milin R. Acharya, Edward A. Sausville, Suoping Zhai, Eunhee W. Woo, Jürgen Venitz, William D. Figg^*, Alex Sparreboom

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

A rapid method was developed for the quantitative determination of the novel histone deacetylase inhibitor, MS-275, in human plasma. Calibration curves were constructed in the range of 1-100ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a one-step protein precipitation with acetonitrile of 0.1ml samples. The analysis was performed on a column (75mm ×4.6mm i.d.) packed with 3.5μm Phenyl-SB material, using methanol - 10mM ammonium formate (55:45 (v/v)) as the mobile phase. The column effluent was monitored by mass spectrometry with positive electrospray ionization. The values for precision and accuracy were always ≤5.58 and <11.4% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of MS-275 in a cancer patient.

Original language	English
Pages (from-to)	289-294
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	804
Issue number	2
DOIs	https://doi.org/10.1016/j.jchromb.2004.01.023
State	Published - 25 May 2004
Externally published	Yes

Keywords

Histone deacetylase inhibitor
MS-275

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10.1016/j.jchromb.2004.01.023

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Hwang, K., Acharya, M. R., Sausville, E. A., Zhai, S., Woo, E. W., Venitz, J., Figg, W. D., & Sparreboom, A. (2004). Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 804(2), 289-294. https://doi.org/10.1016/j.jchromb.2004.01.023

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title = "Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry",

abstract = "A rapid method was developed for the quantitative determination of the novel histone deacetylase inhibitor, MS-275, in human plasma. Calibration curves were constructed in the range of 1-100ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a one-step protein precipitation with acetonitrile of 0.1ml samples. The analysis was performed on a column (75mm ×4.6mm i.d.) packed with 3.5μm Phenyl-SB material, using methanol - 10mM ammonium formate (55:45 (v/v)) as the mobile phase. The column effluent was monitored by mass spectrometry with positive electrospray ionization. The values for precision and accuracy were always ≤5.58 and <11.4% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of MS-275 in a cancer patient.",

keywords = "Histone deacetylase inhibitor, MS-275",

author = "Kyunghwa Hwang and Acharya, {Milin R.} and Sausville, {Edward A.} and Suoping Zhai and Woo, {Eunhee W.} and J{\"u}rgen Venitz and Figg, {William D.} and Alex Sparreboom",

year = "2004",

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doi = "10.1016/j.jchromb.2004.01.023",

language = "English",

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Hwang, K, Acharya, MR, Sausville, EA, Zhai, S, Woo, EW, Venitz, J, Figg, WD & Sparreboom, A 2004, 'Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 804, no. 2, pp. 289-294. https://doi.org/10.1016/j.jchromb.2004.01.023

Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry. / Hwang, Kyunghwa; Acharya, Milin R.; Sausville, Edward A. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 804, No. 2, 25.05.2004, p. 289-294.

Research output: Contribution to journal › Article › peer-review

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T1 - Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry

AU - Hwang, Kyunghwa

AU - Acharya, Milin R.

AU - Sausville, Edward A.

AU - Zhai, Suoping

AU - Woo, Eunhee W.

AU - Venitz, Jürgen

AU - Figg, William D.

AU - Sparreboom, Alex

PY - 2004/5/25

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AB - A rapid method was developed for the quantitative determination of the novel histone deacetylase inhibitor, MS-275, in human plasma. Calibration curves were constructed in the range of 1-100ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a one-step protein precipitation with acetonitrile of 0.1ml samples. The analysis was performed on a column (75mm ×4.6mm i.d.) packed with 3.5μm Phenyl-SB material, using methanol - 10mM ammonium formate (55:45 (v/v)) as the mobile phase. The column effluent was monitored by mass spectrometry with positive electrospray ionization. The values for precision and accuracy were always ≤5.58 and <11.4% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of MS-275 in a cancer patient.

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IS - 2

ER -

Determination of MS-275, a novel histone deacetylase inhibitor, in human plasma by liquid chromatography-electrospray mass spectrometry

Abstract

Keywords

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