TY - JOUR
T1 - Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry
AU - Gardner, Erin R.
AU - Liau, Chi Ting
AU - Chu, Zyting E.
AU - Figg, William D.
AU - Sparreboom, Alex
PY - 2006
Y1 - 2006
N2 - A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 μL) were subjected to liquid-liquid extraction with 1-chlorobutane/ acetonitrile (4:1, v/v), with [2H5]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 X 2.1 mm, 3.5 μm). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously.
AB - A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 μL) were subjected to liquid-liquid extraction with 1-chlorobutane/ acetonitrile (4:1, v/v), with [2H5]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 X 2.1 mm, 3.5 μm). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously.
UR - http://www.scopus.com/inward/record.url?scp=33745889636&partnerID=8YFLogxK
U2 - 10.1002/rcm.2577
DO - 10.1002/rcm.2577
M3 - Article
C2 - 16779869
AN - SCOPUS:33745889636
SN - 0951-4198
VL - 20
SP - 2170
EP - 2174
JO - Rapid Communications in Mass Spectrometry
JF - Rapid Communications in Mass Spectrometry
IS - 14
ER -