Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry

Erin R. Gardner; Chi Ting Liau; Zyting E. Chu; William D. Figg; Alex Sparreboom

doi:10.1002/rcm.2577

Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry

Erin R. Gardner, Chi Ting Liau, Zyting E. Chu, William D. Figg^*, Alex Sparreboom

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 μL) were subjected to liquid-liquid extraction with 1-chlorobutane/ acetonitrile (4:1, v/v), with [²H₅]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 X 2.1 mm, 3.5 μm). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously.

Original language	English
Pages (from-to)	2170-2174
Number of pages	5
Journal	Rapid Communications in Mass Spectrometry
Volume	20
Issue number	14
DOIs	https://doi.org/10.1002/rcm.2577
State	Published - 2006
Externally published	Yes

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title = "Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry",

abstract = "A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 μL) were subjected to liquid-liquid extraction with 1-chlorobutane/ acetonitrile (4:1, v/v), with [2H5]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 X 2.1 mm, 3.5 μm). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously.",

author = "Gardner, {Erin R.} and Liau, {Chi Ting} and Chu, {Zyting E.} and Figg, {William D.} and Alex Sparreboom",

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AU - Liau, Chi Ting

AU - Chu, Zyting E.

AU - Figg, William D.

AU - Sparreboom, Alex

PY - 2006

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AB - A sensitive and specific assay for paclitaxel in plasma has been developed to overcome limitations in previously published assays, using liquid chromatography with tandem mass spectrometric detection. Plasma samples (100 μL) were subjected to liquid-liquid extraction with 1-chlorobutane/ acetonitrile (4:1, v/v), with [2H5]paclitaxel employed as the internal standard. Chromatography was carried out with a Waters SymmetryShield C8 column (50 X 2.1 mm, 3.5 μm). The total run time, including equilibration, was 8 min, using a gradient of acetonitrile and 10 mM ammonium formate, pH 4.0. The assay is accurate and precise over the range of 2-2500 ng/mL and has been successfully applied to study the clinical pharmacokinetics of two formulations of paclitaxel, Genaxol and Genetaxyl, given orally and intravenously.

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Determination of paclitaxel in human plasma following the administration of Genaxol or Genetaxyl by liquid chromatography/tandem mass spectrometry

Abstract

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