Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry

Lokesh Jain; Erin R. Gardner; Jürgen Venitz; Giuseppe Giaccone; Brett E. Houk; William D. Figg

doi:10.1016/j.jchromb.2010.09.017

Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry

Lokesh Jain, Erin R. Gardner, Jürgen Venitz, Giuseppe Giaccone, Brett E. Houk, William D. Figg^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A simple, rapid and sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) analytical method was developed for quantification of Hsp90 inhibitor PF-04928473 in human plasma, following administration of its prodrug, PF-04929113. Sample processing involved protein precipitation by addition of 0.4mL of methanol containing internal standard (PF-04972487) to 50μL volume of plasma sample. Chromatographic separation of PF-04928473 and PF-04972487 was achieved on a Phenomenex ^® Luna C18(2) (2.0mm×50mm, 5μm) column using a gradient elution method with mobile phase solvents: methanol containing 0.1% formic acid and 0.1% formic acid at a flow rate of 0.25mL/min. Detection was performed in electrospray positive ionization mode, monitoring the ion transitions from m/z 465.1→350.1 (PF-04928473) and m/z 447.0→329.1 (PF-04972487). The retention times for PF-04928473 and PF-04972487 were 1.86 and 2.85min, respectively. Calibration curves were generated in the range of 2-2000ng/mL. The accuracy and precision ranged from 94.1 to 99.0% and 86.7 to 97.6%, respectively, which were calculated using quality control samples of three different concentrations analyzed in quintuplicate on four different days.

Original language	English
Pages (from-to)	3187-3192
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	878
Issue number	30
DOIs	https://doi.org/10.1016/j.jchromb.2010.09.017
State	Published - 15 Nov 2010
Externally published	Yes

Keywords

Hsp90 inhibitors
PF-04928473
PF-04929113
SNX-2112
SNX-5422

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10.1016/j.jchromb.2010.09.017

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title = "Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry",

abstract = "A simple, rapid and sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) analytical method was developed for quantification of Hsp90 inhibitor PF-04928473 in human plasma, following administration of its prodrug, PF-04929113. Sample processing involved protein precipitation by addition of 0.4mL of methanol containing internal standard (PF-04972487) to 50μL volume of plasma sample. Chromatographic separation of PF-04928473 and PF-04972487 was achieved on a Phenomenex {\textregistered} Luna C18(2) (2.0mm×50mm, 5μm) column using a gradient elution method with mobile phase solvents: methanol containing 0.1% formic acid and 0.1% formic acid at a flow rate of 0.25mL/min. Detection was performed in electrospray positive ionization mode, monitoring the ion transitions from m/z 465.1→350.1 (PF-04928473) and m/z 447.0→329.1 (PF-04972487). The retention times for PF-04928473 and PF-04972487 were 1.86 and 2.85min, respectively. Calibration curves were generated in the range of 2-2000ng/mL. The accuracy and precision ranged from 94.1 to 99.0% and 86.7 to 97.6%, respectively, which were calculated using quality control samples of three different concentrations analyzed in quintuplicate on four different days.",

keywords = "Hsp90 inhibitors, PF-04928473, PF-04929113, SNX-2112, SNX-5422",

author = "Lokesh Jain and Gardner, {Erin R.} and J{\"u}rgen Venitz and Giuseppe Giaccone and Houk, {Brett E.} and Figg, {William D.}",

note = "Funding Information: This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health , under contract HHSN261200800001E (ERG). This work was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research . ",

year = "2010",

month = nov,

day = "15",

doi = "10.1016/j.jchromb.2010.09.017",

language = "English",

volume = "878",

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journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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T1 - Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry

AU - Jain, Lokesh

AU - Gardner, Erin R.

AU - Venitz, Jürgen

AU - Giaccone, Giuseppe

AU - Houk, Brett E.

AU - Figg, William D.

N1 - Funding Information: This project has been funded in whole or in part with federal funds from the National Cancer Institute, National Institutes of Health , under contract HHSN261200800001E (ERG). This work was supported by the Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research .

PY - 2010/11/15

Y1 - 2010/11/15

N2 - A simple, rapid and sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) analytical method was developed for quantification of Hsp90 inhibitor PF-04928473 in human plasma, following administration of its prodrug, PF-04929113. Sample processing involved protein precipitation by addition of 0.4mL of methanol containing internal standard (PF-04972487) to 50μL volume of plasma sample. Chromatographic separation of PF-04928473 and PF-04972487 was achieved on a Phenomenex ® Luna C18(2) (2.0mm×50mm, 5μm) column using a gradient elution method with mobile phase solvents: methanol containing 0.1% formic acid and 0.1% formic acid at a flow rate of 0.25mL/min. Detection was performed in electrospray positive ionization mode, monitoring the ion transitions from m/z 465.1→350.1 (PF-04928473) and m/z 447.0→329.1 (PF-04972487). The retention times for PF-04928473 and PF-04972487 were 1.86 and 2.85min, respectively. Calibration curves were generated in the range of 2-2000ng/mL. The accuracy and precision ranged from 94.1 to 99.0% and 86.7 to 97.6%, respectively, which were calculated using quality control samples of three different concentrations analyzed in quintuplicate on four different days.

AB - A simple, rapid and sensitive liquid chromatography/tandem mass spectrometric (LC/MS/MS) analytical method was developed for quantification of Hsp90 inhibitor PF-04928473 in human plasma, following administration of its prodrug, PF-04929113. Sample processing involved protein precipitation by addition of 0.4mL of methanol containing internal standard (PF-04972487) to 50μL volume of plasma sample. Chromatographic separation of PF-04928473 and PF-04972487 was achieved on a Phenomenex ® Luna C18(2) (2.0mm×50mm, 5μm) column using a gradient elution method with mobile phase solvents: methanol containing 0.1% formic acid and 0.1% formic acid at a flow rate of 0.25mL/min. Detection was performed in electrospray positive ionization mode, monitoring the ion transitions from m/z 465.1→350.1 (PF-04928473) and m/z 447.0→329.1 (PF-04972487). The retention times for PF-04928473 and PF-04972487 were 1.86 and 2.85min, respectively. Calibration curves were generated in the range of 2-2000ng/mL. The accuracy and precision ranged from 94.1 to 99.0% and 86.7 to 97.6%, respectively, which were calculated using quality control samples of three different concentrations analyzed in quintuplicate on four different days.

KW - Hsp90 inhibitors

KW - PF-04928473

KW - PF-04929113

KW - SNX-2112

KW - SNX-5422

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U2 - 10.1016/j.jchromb.2010.09.017

DO - 10.1016/j.jchromb.2010.09.017

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C2 - 20951100

AN - SCOPUS:78049456442

SN - 1570-0232

VL - 878

SP - 3187

EP - 3192

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

IS - 30

ER -

Determination of PF-04928473 in human plasma using liquid chromatography with tandem mass spectrometry

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Keywords

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