Determination of the cyclic depsipeptide FK228, a histone deacetylase inhibitor, by liquid chromatography-mass spectrometry

Kyunghwa Hwang; Richard L. Piekarz; Susan E. Bates; William D. Figg; Alex Sparreboom

doi:10.1016/j.jchromb.2004.06.007

Determination of the cyclic depsipeptide FK228, a histone deacetylase inhibitor, by liquid chromatography-mass spectrometry

Kyunghwa Hwang, Richard L. Piekarz, Susan E. Bates, William D. Figg^*, Alex Sparreboom

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

An analytical method was developed for the quantitative determination of the novel histone deacetylase inhibitor, depsipeptide FK228 (formerly FR901228; NSC 630176), in human plasma. Calibration curves were constructed in the range of 0.5-100 ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a liquid-liquid extraction with ethyl acetate using 500 μl aliquots of plasma. The analyte was separated on a column (50 mm × 4.6 mm i.d.) packed with 3.5 μm C8 material, and eluted with methanol - 10 mM ammonium formate (55:45; v/v; pH 8). The column effluent was monitored by mass spectrometry with electrospray ionization. The values for precision and accuracy were always ≤7.88% and <3.33% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of FK228 in a cancer patient.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	809
Issue number	1
DOIs	https://doi.org/10.1016/j.jchromb.2004.06.007
State	Published - 25 Sep 2004
Externally published	Yes

Keywords

Cyclic depsipeptide
FK228

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10.1016/j.jchromb.2004.06.007

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title = "Determination of the cyclic depsipeptide FK228, a histone deacetylase inhibitor, by liquid chromatography-mass spectrometry",

abstract = "An analytical method was developed for the quantitative determination of the novel histone deacetylase inhibitor, depsipeptide FK228 (formerly FR901228; NSC 630176), in human plasma. Calibration curves were constructed in the range of 0.5-100 ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a liquid-liquid extraction with ethyl acetate using 500 μl aliquots of plasma. The analyte was separated on a column (50 mm × 4.6 mm i.d.) packed with 3.5 μm C8 material, and eluted with methanol - 10 mM ammonium formate (55:45; v/v; pH 8). The column effluent was monitored by mass spectrometry with electrospray ionization. The values for precision and accuracy were always ≤7.88% and <3.33% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of FK228 in a cancer patient.",

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doi = "10.1016/j.jchromb.2004.06.007",

language = "English",

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journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

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Determination of the cyclic depsipeptide FK228, a histone deacetylase inhibitor, by liquid chromatography-mass spectrometry. / Hwang, Kyunghwa; Piekarz, Richard L.; Bates, Susan E. et al.
In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 809, No. 1, 25.09.2004, p. 81-86.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Determination of the cyclic depsipeptide FK228, a histone deacetylase inhibitor, by liquid chromatography-mass spectrometry

AU - Hwang, Kyunghwa

AU - Piekarz, Richard L.

AU - Bates, Susan E.

AU - Figg, William D.

AU - Sparreboom, Alex

PY - 2004/9/25

Y1 - 2004/9/25

N2 - An analytical method was developed for the quantitative determination of the novel histone deacetylase inhibitor, depsipeptide FK228 (formerly FR901228; NSC 630176), in human plasma. Calibration curves were constructed in the range of 0.5-100 ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a liquid-liquid extraction with ethyl acetate using 500 μl aliquots of plasma. The analyte was separated on a column (50 mm × 4.6 mm i.d.) packed with 3.5 μm C8 material, and eluted with methanol - 10 mM ammonium formate (55:45; v/v; pH 8). The column effluent was monitored by mass spectrometry with electrospray ionization. The values for precision and accuracy were always ≤7.88% and <3.33% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of FK228 in a cancer patient.

AB - An analytical method was developed for the quantitative determination of the novel histone deacetylase inhibitor, depsipeptide FK228 (formerly FR901228; NSC 630176), in human plasma. Calibration curves were constructed in the range of 0.5-100 ng/ml, and were analyzed using a weight factor proportional to the nominal concentration. Sample pretreatment involved a liquid-liquid extraction with ethyl acetate using 500 μl aliquots of plasma. The analyte was separated on a column (50 mm × 4.6 mm i.d.) packed with 3.5 μm C8 material, and eluted with methanol - 10 mM ammonium formate (55:45; v/v; pH 8). The column effluent was monitored by mass spectrometry with electrospray ionization. The values for precision and accuracy were always ≤7.88% and <3.33% relative error, respectively. The method was successfully applied to examine the pharmacokinetics of FK228 in a cancer patient.

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IS - 1

ER -

Determination of the cyclic depsipeptide FK228, a histone deacetylase inhibitor, by liquid chromatography-mass spectrometry

Abstract

Keywords

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