Developing a toolbox for analysis of warrior wound biopsies: Vibrational spectroscopy

Nicole J. Crane, Frederick P. O'Brien, Jonathan A. Forsberg, Benjamin K. Potter, Eric A. Elster

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations


The management of modern traumatic war wounds remains a significant challenge for clinicians. This is a reflection of the extensive osseous and soft-tissue damage caused by blasts and high-energy projectiles. The ensuing inflammatory response ultimately dictates the pace of wound healing and tissue regeneration. Consequently, the eventual timing of wound closure or definitive coverage is often subjectively based. Some wounds require an extended period of time to close or fail to remain closed, despite the use and application of novel wound-specific treatment modalities. Aside from impaired wound healing, additional wound complications include wound infection, biofilm formation, and heterotopic ossification (the pathological mineralization of soft tissues). An understanding of the molecular environment of acute wounds throughout the debridement process can provide valuable insight into the mechanisms associated with the eventual wound outcome. The analysis of Raman spectra of ex vivo wound biopsy tissue obtained from serial traumatic wound debridements reveals a decreased 1665 cm-1/1445 cm-1 band area ratio in impaired healing wounds, indicative of an impaired remodeling process, in addition to a decreased 1240 cm-1/1270cm-1. The examination of debrided tissue exhibits mineralization during the early development of heterotopic ossification. Finally, preliminary results suggest that Fourier transform infrared (FT-IR) images of wound effluent may be able to provide early microbiological information about the wound.

Original languageEnglish
Title of host publicationOptical Biopsy IX
StatePublished - 2011
Externally publishedYes
EventOptical Biopsy IX - San Francisco, CA, United States
Duration: 24 Jan 201126 Jan 2011

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


ConferenceOptical Biopsy IX
Country/TerritoryUnited States
CitySan Francisco, CA


  • FT-IR imaging
  • Raman spectroscopy
  • biopsies
  • combat wounds
  • wound effluent


Dive into the research topics of 'Developing a toolbox for analysis of warrior wound biopsies: Vibrational spectroscopy'. Together they form a unique fingerprint.

Cite this