Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia

Matthew B. Givens; Jessica T. Lin; Chanthap Lon; Panita Gosi; Meng Chuor Char; Charlotte A. Lanteri; David L. Saunders; Jonathan J. Julianoa

doi:10.1128/JCM.02274-13

Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia

Matthew B. Givens, Jessica T. Lin^*, Chanthap Lon, Panita Gosi, Meng Chuor Char, Charlotte A. Lanteri, David L. Saunders, Jonathan J. Julianoa

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

A heteroduplex tracking assay used to genotype Plasmodium vivax merozoite surface protein 1 was adapted to a capillary electrophoresis format, obviating the need for radiolabeled probes and allowing its use in settings where malaria is endemic. This new assay achieved good allelic discrimination and detected high multiplicities of infection in 63 P. vivax infections in Cambodia. More than half of the recurrent parasitemias sampled displayed identical or highly related genotypes compared to the initial genotype, suggesting that they represented relapses.

Original language	English
Pages (from-to)	298-301
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	52
Issue number	1
DOIs	https://doi.org/10.1128/JCM.02274-13
State	Published - Jan 2014
Externally published	Yes

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Givens, M. B., Lin, J. T., Lon, C., Gosi, P., Char, M. C., Lanteri, C. A., Saunders, D. L., & Julianoa, J. J. (2014). Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia. Journal of Clinical Microbiology, 52(1), 298-301. https://doi.org/10.1128/JCM.02274-13

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title = "Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia",

abstract = "A heteroduplex tracking assay used to genotype Plasmodium vivax merozoite surface protein 1 was adapted to a capillary electrophoresis format, obviating the need for radiolabeled probes and allowing its use in settings where malaria is endemic. This new assay achieved good allelic discrimination and detected high multiplicities of infection in 63 P. vivax infections in Cambodia. More than half of the recurrent parasitemias sampled displayed identical or highly related genotypes compared to the initial genotype, suggesting that they represented relapses.",

author = "Givens, {Matthew B.} and Lin, {Jessica T.} and Chanthap Lon and Panita Gosi and Char, {Meng Chuor} and Lanteri, {Charlotte A.} and Saunders, {David L.} and Julianoa, {Jonathan J.}",

year = "2014",

month = jan,

doi = "10.1128/JCM.02274-13",

language = "English",

volume = "52",

pages = "298--301",

journal = "Journal of Clinical Microbiology",

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Givens, MB, Lin, JT, Lon, C, Gosi, P, Char, MC, Lanteri, CA, Saunders, DL & Julianoa, JJ 2014, 'Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia', Journal of Clinical Microbiology, vol. 52, no. 1, pp. 298-301. https://doi.org/10.1128/JCM.02274-13

Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia. / Givens, Matthew B.; Lin, Jessica T.; Lon, Chanthap et al.
In: Journal of Clinical Microbiology, Vol. 52, No. 1, 01.2014, p. 298-301.

Research output: Contribution to journal › Article › peer-review

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AU - Givens, Matthew B.

AU - Lin, Jessica T.

AU - Lon, Chanthap

AU - Gosi, Panita

AU - Char, Meng Chuor

AU - Lanteri, Charlotte A.

AU - Saunders, David L.

AU - Julianoa, Jonathan J.

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Development of a capillary electrophoresis-based heteroduplex tracking assay to measure in-host genetic diversity of initial and recurrent plasmodium vivax infections in cambodia

Abstract

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