@article{7bf01636667b4978b4ec5aa03b225e93,
title = "Development of a hybrid alphavirus-SARS-CoV-2 pseudovirion for rapid quantification of neutralization antibodies and antiviral drugs",
abstract = "Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike protein (S)-pseudotyped viruses are commonly used for quantifying antiviral drugs and neutralizing antibodies. Here, we describe the development of a hybrid alphavirus-SARS-CoV-2 (Ha-CoV-2) pseudovirion, which is a non-replicating SARS-CoV-2 virus-like particle composed of viral structural proteins (S, M, N, and E) and an RNA genome derived from a fast-expressing alphaviral vector. We validated Ha-CoV-2 for rapid quantification of neutralization antibodies, antiviral drugs, and viral variants. In addition, as a proof of concept, we used Ha-CoV-2 to quantify the neutralizing antibodies from an infected and vaccinated individual and found that the one-dose vaccination with Moderna mRNA-1273 greatly increased the anti-serum titer by approximately 6-fold. The post-vaccination serum can neutralize all nine variants tested. These results demonstrate that Ha-CoV-2 can be used as a robust platform for the rapid quantification of neutralizing antibodies against SARS-CoV-2 and its emerging variants.",
keywords = "COVID-19, Ha-CoV-2, SARS-CoV-2, SARS-CoV-2 variants, alphavirus, antiviral drug, coronavirus, lentivirus, mRNA vaccine, neutralizing antibody, pseudovirus",
author = "Brian Hetrick and Chilin, {Linda D.} and Sijia He and Deemah Dabbagh and Farhang Alem and Aarthi Narayanan and Alessandra Luchini and Tuanjie Li and Xuefeng Liu and Joshua Copeland and Angela Pak and Tshaka Cunningham and Lance Liotta and Petricoin, {Emanuel F.} and Ali Andalibi and Yuntao Wu",
note = "Funding Information: The authors wish to thank Janice Yoon for technical assistance and Dr. Pei-Hui Wang for providing FLAG-tagged M, N, and E expression vectors. This work was supported by the George Mason University internal research fund. Experiments were designed by B.H. and Y.W. Manuscript was written by Y.W. Experiments were performed by B.H. L.D.C. S.H. D.D. F.A. A.N. A.L. T.L. X.L. J.C. A.P. T.C. L.L, E.F.P. and A.A. A patent application has been filed by George Mason University and is licensed for product development. Y.W. is a founder of Virongy Biosciences, Inc. and a member of its advisory board. T.C. is the chief scientific officer of Polaris Genomics, Inc. Funding Information: The authors wish to thank Janice Yoon for technical assistance and Dr. Pei-Hui Wang for providing FLAG-tagged M, N, and E expression vectors. This work was supported by the George Mason University internal research fund. Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
month = mar,
day = "28",
doi = "10.1016/j.crmeth.2022.100181",
language = "English",
volume = "2",
journal = "Cell Reports Methods",
issn = "2667-2375",
number = "3",
}