Development of a novel proteomic approach for mitochondrial proteomics from cardiac tissue from patients with atrial fibrillation

Maryam Goudarzi, Mark M. Ross, Weidong Zhou, Amy Van Meter, Jianghong Deng, Lisa M. Martin, Chidima Martin, Lance Liotta, Emanuel Petricoin, Niv Ad*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

19 Scopus citations


Atrial fibrillation (AF) is the most common cardiac arrhythmia affecting approximately 2.2 million Americans. Because several studies have suggested that changes in mitochondrial function and morphology may contribute to AF, we developed a novel proteomic workflow focused on the identification of differentially expressed mitochondrial proteins in AF patients. Right human atrial tissue was collected from 20 patients, 10 with and 10 without AF, and the tissue was subjected to hydrostatic pressure cycling-based lysis followed by label-free mass spectrometric (MS) analysis of mitochondrial enriched isolates. Approximately 5% of the 700 proteins identified by MS analysis were differentially expressed between the AF and non-AF samples. We chose four differentially abundant proteins for further verification using reverse phase protein microarray analysis based on their known importance in energy production and regulatory association with atrial ion channels: four and a half LIM, destrin, heat shock protein 2, and chaperonin-containing TCP1. These initial study results provide evidence that a workflow to identify AF-related proteins that combines a powerful upfront tissue cell lysis with high resolution MS for discovery and protein array technology for verification may be an effective strategy for discovering candidate markers in highly fibrous tissue samples.

Original languageEnglish
Pages (from-to)3484-3492
Number of pages9
JournalJournal of Proteome Research
Issue number8
StatePublished - 5 Aug 2011
Externally publishedYes


  • CCT5
  • FHL2
  • HSP27
  • atrial fibrillation
  • destrin
  • hydrostatic pressure cycling
  • mass spectrometry
  • mitochondria
  • proteomics
  • reverse-phase protein microarray


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