Development of a pan-serotype reverse transcription loop-mediated isothermal amplification assay for the detection of dengue virus

Allison Dauner*, Indrani Mitra, Theron Gilliland, Sajeewane Seales, Subhamoy Pal, Shih-Chun Yang, Carolina Guevara, Yung-Chuan Liu, Tadeusz Kochel, Shuenn-Jue Wu

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations

Abstract

During dengue outbreaks, acute diagnosis at the patient's point of need followed by appropriate supportive therapy reduces morbidity and mortality. To facilitate needed diagnosis, we developed and optimized a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay that detects all 4 serotypes of dengue virus (DENV). We used a quencher to reduce nonspecific amplification. The assay does not require expensive thermocyclers, utilizing a simple water bath to maintain the reaction at 63 °C. Results can be visualized using UV fluorescence, handheld readers, or lateral flow immunochromatographic tests. We report a sensitivity of 86.3% (95% confidence interval [CI], 72.7–94.8%) and specificity of 93.0% (95% CI, 83.0–98.1%) using a panel of clinical specimens characterized by DENV quantitative reverse transcription–polymerase chain reaction. This pan-serotype DENV RT-LAMP can be …
Original languageAmerican English
Pages (from-to)30-36
JournalDiagnostic Microbiology and Infectious Disease
Volume83
Issue number1
StatePublished - 1 Sep 2015

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