Development of High-Throughput Mass Spectrometry-Based Approaches for Cancer Biomarker Discovery and Implementation

Brian L. Hood, Nicolas A.S. Stewart, Thomas P. Conrads*

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

11 Scopus citations

Abstract

A major goal of cancer research is elucidating the molecular events underlying carcinogenesis, with the goal of discovering better diagnostic markers and therapeutic targets. Proteomics aims to facilitate this process by applying newly developed methods and advanced analytic tools, such as mass spectrometry, for the investigation of the protein complement en masse. Proteomics is the comprehensive study of proteins and is aimed at analyzing their structure, function, modifications, expression, interactions, and localization in complex biological systems. This article reviews the state-of-the art in mass spectrometry-based approaches and their application for cancer biomarker discovery and validation.

Original languageEnglish
Pages (from-to)115-138
Number of pages24
JournalClinics in Laboratory Medicine
Volume29
Issue number1
DOIs
StatePublished - Mar 2009
Externally publishedYes

Keywords

  • Biomarker
  • Discovery
  • Mass spectrometry
  • Proteomics
  • Validation

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