TY - JOUR
T1 - Differential allele expression of host defense genes, pulmonary surfactant protein-A and Osteopontin, in rat
AU - Lin, Zhenwu
AU - Wang, Yunhua
AU - Zhu, Kangmin
AU - Floros, Joanna
N1 - Funding Information:
The authors thank Jelena Pavlovic for her work in gel scanning and quantification of allele products with ImageQuant software, Jeff Sundstrom for his initial work on OPN allele expression, and Drs. D.T. Denhardt, and S.R. Rittling for valuable suggestions. This work was supported by NIH R37HL34788, AHA 198312P, and Dean’s Feasibility Grant of The Pennsylvania State University College of Medicine.
PY - 2004/11
Y1 - 2004/11
N2 - Differential allele-specific expression has been observed in several genes involved in immunity. SP-A and OPN play a role in innate host defense. To determine whether SP-A and OPN are subject to differential allele-specific regulation, we investigated their gene or allele-specific expression in various tissues. The results showed: (1) Tissue-specific expression with high levels in lung (SP-A) and kidney (OPN). (2) Differences in allele-specific expression among individuals and tissues. SP-A showed an exclusively balanced biallelic expression (BB) in lung, but both BB and imbalanced biallelic (IB) expression in colon. Allele expression of OPN was more heterogeneous, e.g. in colon BB (22%), IB (64%), and monoallelic expression (MO) (14%). (3) Differential allele-specific expression was observed in all tissues studied (OPN) or in all extrapulmonary tissues (SP-A). (4) Family studies indicated that inheritable factor(s) may be involved in the regulation of allele-specific expression. (5) Analysis of co-expression of gene-specific alleles from double heterozygous rats revealed lack of coordinate allele expression among SP-A, SP-D, and OPN. We conclude that allele-specific expression occurs among genes of innate host defense. This may yet provide another level of regulatory complexity for molecules involved in the first line of defense.
AB - Differential allele-specific expression has been observed in several genes involved in immunity. SP-A and OPN play a role in innate host defense. To determine whether SP-A and OPN are subject to differential allele-specific regulation, we investigated their gene or allele-specific expression in various tissues. The results showed: (1) Tissue-specific expression with high levels in lung (SP-A) and kidney (OPN). (2) Differences in allele-specific expression among individuals and tissues. SP-A showed an exclusively balanced biallelic expression (BB) in lung, but both BB and imbalanced biallelic (IB) expression in colon. Allele expression of OPN was more heterogeneous, e.g. in colon BB (22%), IB (64%), and monoallelic expression (MO) (14%). (3) Differential allele-specific expression was observed in all tissues studied (OPN) or in all extrapulmonary tissues (SP-A). (4) Family studies indicated that inheritable factor(s) may be involved in the regulation of allele-specific expression. (5) Analysis of co-expression of gene-specific alleles from double heterozygous rats revealed lack of coordinate allele expression among SP-A, SP-D, and OPN. We conclude that allele-specific expression occurs among genes of innate host defense. This may yet provide another level of regulatory complexity for molecules involved in the first line of defense.
KW - BB
KW - IB
KW - IB A (IB C or IB G)
KW - IB with higher level of expression of allele A (C or G)
KW - MO
KW - MO A (MO C or MO G)
KW - OPN
KW - Osteopontin
KW - SP-A
KW - balanced biallelic expression
KW - imbalanced biallelic expression
KW - monoallelic expression
KW - only allele A (C or G) is expressed
UR - http://www.scopus.com/inward/record.url?scp=5144228628&partnerID=8YFLogxK
U2 - 10.1016/j.molimm.2004.06.006
DO - 10.1016/j.molimm.2004.06.006
M3 - Article
C2 - 15482851
AN - SCOPUS:5144228628
SN - 0161-5890
VL - 41
SP - 1155
EP - 1165
JO - Molecular Immunology
JF - Molecular Immunology
IS - 12
ER -