The differential in-gel electrophoresis (2D-DIGE) was used to compare protein expression profiles in protein homogenates from biopsy samples to detect disease related expression changes, and separate them from changes caused by natural cellular heterogeneity. Laser microdissection was performed on a Leica AS LMD instrument. Proteins from frozen biopsy samples were isolated following homogenization in buffer, labeled with Cy dyes and focused on IPG strips. Detectable differences were observed in each comparison, specially between intraductal hyperplasia vs. ductal carcinoma.
|Number of pages||2|
|State||Published - 2002|
|Event||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States|
Duration: 2 Jun 2002 → 6 Jun 2002
|Conference||Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics|
|Period||2/06/02 → 6/06/02|