Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS

Anthony G. Sullivan; S. Russell; C. Arciero; B. Deyarmin; J. Hooke; C. Shriver; R. I. Somiari

Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS

Anthony G. Sullivan^*, S. Russell, C. Arciero, B. Deyarmin, J. Hooke, C. Shriver, R. I. Somiari

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

The differential in-gel electrophoresis (2D-DIGE) was used to compare protein expression profiles in protein homogenates from biopsy samples to detect disease related expression changes, and separate them from changes caused by natural cellular heterogeneity. Laser microdissection was performed on a Leica AS LMD instrument. Proteins from frozen biopsy samples were isolated following homogenization in buffer, labeled with Cy dyes and focused on IPG strips. Detectable differences were observed in each comparison, specially between intraductal hyperplasia vs. ductal carcinoma.

Original language	English
Pages	231-232
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

Sullivan, A. G., Russell, S., Arciero, C., Deyarmin, B., Hooke, J., Shriver, C., & Somiari, R. I. (2002). Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS. 231-232. Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

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title = "Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS",

abstract = "The differential in-gel electrophoresis (2D-DIGE) was used to compare protein expression profiles in protein homogenates from biopsy samples to detect disease related expression changes, and separate them from changes caused by natural cellular heterogeneity. Laser microdissection was performed on a Leica AS LMD instrument. Proteins from frozen biopsy samples were isolated following homogenization in buffer, labeled with Cy dyes and focused on IPG strips. Detectable differences were observed in each comparison, specially between intraductal hyperplasia vs. ductal carcinoma.",

author = "Sullivan, {Anthony G.} and S. Russell and C. Arciero and B. Deyarmin and J. Hooke and C. Shriver and Somiari, {R. I.}",

year = "2002",

language = "English",

pages = "231--232",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

Sullivan, AG, Russell, S, Arciero, C, Deyarmin, B , Hooke, J , Shriver, C & Somiari, RI 2002, 'Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS', Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States, 2/06/02 - 6/06/02 pp. 231-232.

Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS. / Sullivan, Anthony G.; Russell, S.; Arciero, C. et al.
2002. 231-232 Paper presented at Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics, Orlando, FL, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS

AU - Sullivan, Anthony G.

AU - Russell, S.

AU - Arciero, C.

AU - Deyarmin, B.

AU - Hooke, J.

AU - Shriver, C.

AU - Somiari, R. I.

PY - 2002

Y1 - 2002

N2 - The differential in-gel electrophoresis (2D-DIGE) was used to compare protein expression profiles in protein homogenates from biopsy samples to detect disease related expression changes, and separate them from changes caused by natural cellular heterogeneity. Laser microdissection was performed on a Leica AS LMD instrument. Proteins from frozen biopsy samples were isolated following homogenization in buffer, labeled with Cy dyes and focused on IPG strips. Detectable differences were observed in each comparison, specially between intraductal hyperplasia vs. ductal carcinoma.

AB - The differential in-gel electrophoresis (2D-DIGE) was used to compare protein expression profiles in protein homogenates from biopsy samples to detect disease related expression changes, and separate them from changes caused by natural cellular heterogeneity. Laser microdissection was performed on a Leica AS LMD instrument. Proteins from frozen biopsy samples were isolated following homogenization in buffer, labeled with Cy dyes and focused on IPG strips. Detectable differences were observed in each comparison, specially between intraductal hyperplasia vs. ductal carcinoma.

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M3 - Paper

AN - SCOPUS:2442652842

SP - 231

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T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Differential protein expression analysis in human breast cancer using tissue samples and pure cell populations obtained by LMD using 2D-DIGE, MALDI and Q-Tof MS/MS

Abstract

Conference

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