Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4+ T cells

Richard G. Carroll; James L. Riley; Bruce L. Levine; Yu Feng; Sumesh Kaushal; David W. Ritchey; Wendy Bernstein; Owen S. Weislow; Charles R. Brown; Edward A. Berger; Carl H. June; Daniel C. St. Louis

doi:10.1126/science.276.5310.273

Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4⁺ T cells

Richard G. Carroll, James L. Riley, Bruce L. Levine, Yu Feng, Sumesh Kaushal, David W. Ritchey, Wendy Bernstein, Owen S. Weislow, Charles R. Brown, Edward A. Berger, Carl H. June^*, Daniel C. St. Louis

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

180 Scopus citations

Abstract

Activation of CD4⁺ T lymphocytes from human immunodeficiency virus- type 1 (HIV-1)-infected donors with immobilized antibodies to CD3 and CD28 induces a virus-resistant state. This effect is specific for macrophage- tropic HIV-1. Transcripts encoding CXCR4/Fusin, the fusion cofactor used by T cell line-tropic isolates, were abundant in CD3/CD28-stimulated cells, but transcripts encoding CCR5, the fusion cofactor used by macrophage-tropic viruses, were not detectable. Thus, CD3/CD28 costimulation induces an HIV-1- resistant phenotype similar to that seen in some highly exposed and HIV- uninfected individuals.

Original language	English
Pages (from-to)	273-276
Number of pages	4
Journal	Science
Volume	276
Issue number	5310
DOIs	https://doi.org/10.1126/science.276.5310.273
State	Published - 11 Apr 1997
Externally published	Yes

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@article{e685e390700f4ba99f80596fb2e16fa3,

title = "Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4+ T cells",

abstract = "Activation of CD4+ T lymphocytes from human immunodeficiency virus- type 1 (HIV-1)-infected donors with immobilized antibodies to CD3 and CD28 induces a virus-resistant state. This effect is specific for macrophage- tropic HIV-1. Transcripts encoding CXCR4/Fusin, the fusion cofactor used by T cell line-tropic isolates, were abundant in CD3/CD28-stimulated cells, but transcripts encoding CCR5, the fusion cofactor used by macrophage-tropic viruses, were not detectable. Thus, CD3/CD28 costimulation induces an HIV-1- resistant phenotype similar to that seen in some highly exposed and HIV- uninfected individuals.",

author = "Carroll, {Richard G.} and Riley, {James L.} and Levine, {Bruce L.} and Yu Feng and Sumesh Kaushal and Ritchey, {David W.} and Wendy Bernstein and Weislow, {Owen S.} and Brown, {Charles R.} and Berger, {Edward A.} and June, {Carl H.} and {St. Louis}, {Daniel C.}",

year = "1997",

month = apr,

day = "11",

doi = "10.1126/science.276.5310.273",

language = "English",

volume = "276",

pages = "273--276",

journal = "Science",

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T1 - Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4+ T cells

AU - Carroll, Richard G.

AU - Riley, James L.

AU - Levine, Bruce L.

AU - Feng, Yu

AU - Kaushal, Sumesh

AU - Ritchey, David W.

AU - Bernstein, Wendy

AU - Weislow, Owen S.

AU - Brown, Charles R.

AU - Berger, Edward A.

AU - June, Carl H.

AU - St. Louis, Daniel C.

PY - 1997/4/11

Y1 - 1997/4/11

N2 - Activation of CD4+ T lymphocytes from human immunodeficiency virus- type 1 (HIV-1)-infected donors with immobilized antibodies to CD3 and CD28 induces a virus-resistant state. This effect is specific for macrophage- tropic HIV-1. Transcripts encoding CXCR4/Fusin, the fusion cofactor used by T cell line-tropic isolates, were abundant in CD3/CD28-stimulated cells, but transcripts encoding CCR5, the fusion cofactor used by macrophage-tropic viruses, were not detectable. Thus, CD3/CD28 costimulation induces an HIV-1- resistant phenotype similar to that seen in some highly exposed and HIV- uninfected individuals.

AB - Activation of CD4+ T lymphocytes from human immunodeficiency virus- type 1 (HIV-1)-infected donors with immobilized antibodies to CD3 and CD28 induces a virus-resistant state. This effect is specific for macrophage- tropic HIV-1. Transcripts encoding CXCR4/Fusin, the fusion cofactor used by T cell line-tropic isolates, were abundant in CD3/CD28-stimulated cells, but transcripts encoding CCR5, the fusion cofactor used by macrophage-tropic viruses, were not detectable. Thus, CD3/CD28 costimulation induces an HIV-1- resistant phenotype similar to that seen in some highly exposed and HIV- uninfected individuals.

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U2 - 10.1126/science.276.5310.273

DO - 10.1126/science.276.5310.273

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JO - Science

JF - Science

IS - 5310

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Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4+ T cells

Abstract

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Differential regulation of HIV-1 fusion cofactor expression by CD28 costimulation of CD4⁺ T cells