TY - JOUR
T1 - DNA bending is induced in an enhancer by the DNA-binding domain of the bovine papillomavirus E2 protein
AU - Moskaluk, C.
AU - Bastia, D.
PY - 1988
Y1 - 1988
N2 - The E2 gene of bovine papillomavirus type 1 has been shown to encode a DNA-binding protein and to trans-activate the viral enhancer. We have localized the DNA-binding domain of the E2 protein to the carboxyl-terminal 126 amino acids of the E2 open reading frame. The DNA-binding domain has been expressed in Escherichia coli and partially purified. Gel retardation and DNase 1 'footprinting' on the bovine papillomavirus type 1 enhancer identify the sequence motif ACCN6GGT (in which N = any nucleotide) as the E2 binding site. Using electrophoretic methods we have shown that the DNA-binding domain changes conformation of the enhancer by inducing significant DNA bending.
AB - The E2 gene of bovine papillomavirus type 1 has been shown to encode a DNA-binding protein and to trans-activate the viral enhancer. We have localized the DNA-binding domain of the E2 protein to the carboxyl-terminal 126 amino acids of the E2 open reading frame. The DNA-binding domain has been expressed in Escherichia coli and partially purified. Gel retardation and DNase 1 'footprinting' on the bovine papillomavirus type 1 enhancer identify the sequence motif ACCN6GGT (in which N = any nucleotide) as the E2 binding site. Using electrophoretic methods we have shown that the DNA-binding domain changes conformation of the enhancer by inducing significant DNA bending.
KW - DNA-protein interaction
KW - transcriptional control
UR - http://www.scopus.com/inward/record.url?scp=0024121466&partnerID=8YFLogxK
U2 - 10.1073/pnas.85.6.1826
DO - 10.1073/pnas.85.6.1826
M3 - Article
C2 - 2831538
AN - SCOPUS:0024121466
SN - 0027-8424
VL - 85
SP - 1826
EP - 1830
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -