Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR

Masahiko Yamaguchi; Carl W. Dieffenbach; Ray Connolly; David F. Cruess; Wendy Baur; John B. Sharefkin

doi:10.1101/gr.1.4.286

Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A₂₆₀/A₂₈₀ and on accuracy of mRNA quantitation by reverse transcriptase-PCR

Masahiko Yamaguchi^*, Carl W. Dieffenbach, Ray Connolly, David F. Cruess, Wendy Baur, John B. Sharefkin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Original language	English
Pages (from-to)	286-290
Number of pages	5
Journal	Genome Research
Volume	1
Issue number	4
DOIs	https://doi.org/10.1101/gr.1.4.286
State	Published - 1992
Externally published	Yes

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10.1101/gr.1.4.286

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title = "Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR",

author = "Masahiko Yamaguchi and Dieffenbach, {Carl W.} and Ray Connolly and Cruess, {David F.} and Wendy Baur and Sharefkin, {John B.}",

year = "1992",

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AU - Connolly, Ray

AU - Cruess, David F.

AU - Baur, Wendy

AU - Sharefkin, John B.

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JO - Genome Research

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ER -

Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR

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Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A₂₆₀/A₂₈₀ and on accuracy of mRNA quantitation by reverse transcriptase-PCR