Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR

Masahiko Yamaguchi; Carl W. Dieffenbach; Ray Connolly; David F. Cruess; Wendy Baur; John B. Sharefkin

doi:10.1101/gr.1.4.286

Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A₂₆₀/A₂₈₀ and on accuracy of mRNA quantitation by reverse transcriptase-PCR

Masahiko Yamaguchi^*, Carl W. Dieffenbach, Ray Connolly, David F. Cruess, Wendy Baur, John B. Sharefkin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Original language	English
Pages (from-to)	286-290
Number of pages	5
Journal	Genome Research
Volume	1
Issue number	4
DOIs	https://doi.org/10.1101/gr.1.4.286
State	Published - 1992
Externally published	Yes

Access to Document

10.1101/gr.1.4.286

Cite this

@article{20dc66068e594d8896dba7679b070242,

title = "Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR",

author = "Masahiko Yamaguchi and Dieffenbach, {Carl W.} and Ray Connolly and Cruess, {David F.} and Wendy Baur and Sharefkin, {John B.}",

year = "1992",

doi = "10.1101/gr.1.4.286",

language = "English",

volume = "1",

pages = "286--290",

journal = "Genome Research",

issn = "1088-9051",

publisher = "Cold Spring Harbor Laboratory Press",

number = "4",

}

TY - JOUR

T1 - Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR

AU - Yamaguchi, Masahiko

AU - Dieffenbach, Carl W.

AU - Connolly, Ray

AU - Cruess, David F.

AU - Baur, Wendy

AU - Sharefkin, John B.

PY - 1992

Y1 - 1992

UR - http://www.scopus.com/inward/record.url?scp=0026865191&partnerID=8YFLogxK

U2 - 10.1101/gr.1.4.286

DO - 10.1101/gr.1.4.286

M3 - Article

C2 - 1282433

AN - SCOPUS:0026865191

SN - 1088-9051

VL - 1

SP - 286

EP - 290

JO - Genome Research

JF - Genome Research

IS - 4

ER -

Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A260/A280 and on accuracy of mRNA quantitation by reverse transcriptase-PCR

Access to Document

Fingerprint

Cite this

Effect of different laboratory techniques for guanidinium-phenol-chloroform RNA extraction on A₂₆₀/A₂₈₀ and on accuracy of mRNA quantitation by reverse transcriptase-PCR