Effect of priming with a thymus-independent antigen on susceptibility to B-Cell tolerance

Xiao Rui Yao; David W. Scott

doi:10.1016/0008-8749(92)90303-7

Effect of priming with a thymus-independent antigen on susceptibility to B-Cell tolerance

Xiao Rui Yao, David W. Scott^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of priming on the susceptibility of B-cell subsets to tolerance induction have been tested in a model system in which anti-immunoglobulin (anti-Ig) has been employed as a surrogate for tolerogen. T-cell-depleted B cells were primed in vitro with fluorescein or trinitrophenylated Ficoll (a thymus-independent (TI) antigen) and then exposed overnight to anti-Ig to attempt to induce B-cell anergy. Primed cells were relatively resistant to this tolerance protocol and resistance was hapten specific. The dose response and kinetics suggested that this process was not due to receptor blockade or modulation, but was an active process. Moreover, this priming for resistance to tolerance was reproduced in vivo upon intraperitoneal treatment with haptenated Ficoll. Such in vivo priming for tolerance resistance was long-lasting and did not occur with a thymus-dependent priming protocol with fluoresceinated hemocyanin. These results are discussed in terms of TI priming to drive B cells into cycle and express novel functional and phenotypic properties.

Original language	English
Pages (from-to)	434-443
Number of pages	10
Journal	Cellular Immunology
Volume	142
Issue number	2
DOIs	https://doi.org/10.1016/0008-8749(92)90303-7
State	Published - Jul 1992
Externally published	Yes

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@article{01fb85f678c343838a3fee233e10c68e,

title = "Effect of priming with a thymus-independent antigen on susceptibility to B-Cell tolerance",

abstract = "The effects of priming on the susceptibility of B-cell subsets to tolerance induction have been tested in a model system in which anti-immunoglobulin (anti-Ig) has been employed as a surrogate for tolerogen. T-cell-depleted B cells were primed in vitro with fluorescein or trinitrophenylated Ficoll (a thymus-independent (TI) antigen) and then exposed overnight to anti-Ig to attempt to induce B-cell anergy. Primed cells were relatively resistant to this tolerance protocol and resistance was hapten specific. The dose response and kinetics suggested that this process was not due to receptor blockade or modulation, but was an active process. Moreover, this priming for resistance to tolerance was reproduced in vivo upon intraperitoneal treatment with haptenated Ficoll. Such in vivo priming for tolerance resistance was long-lasting and did not occur with a thymus-dependent priming protocol with fluoresceinated hemocyanin. These results are discussed in terms of TI priming to drive B cells into cycle and express novel functional and phenotypic properties.",

author = "Yao, {Xiao Rui} and Scott, {David W.}",

note = "Funding Information: {\textquoteright} Supported by a grant from the U.S.P.H.S. Grant (AI-2969 1) and the Council for Tobacco Research (No. 2493). This is publication No. 84 from the Immunology Division, University of Rochester Cancer Center. {\textquoteright} To whom correspondence should be addressed at Box 704, University of Rochester Cancer Center, 601 Elmwood Ave., Rochester, NY 14642. 3 Abbreviations used: FL-&I, FL-coupled to Brucella abortus; HEM, Hepes-buffered medium; KLH, keyhole limpet hemocyanin; LPS, Escherichia coli lipopolysaccharide; immunoglobulin; TD, thymus dependent; TI, thymus independent.",

year = "1992",

month = jul,

doi = "10.1016/0008-8749(92)90303-7",

language = "English",

volume = "142",

pages = "434--443",

journal = "Cellular Immunology",

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AU - Yao, Xiao Rui

AU - Scott, David W.

N1 - Funding Information: ’ Supported by a grant from the U.S.P.H.S. Grant (AI-2969 1) and the Council for Tobacco Research (No. 2493). This is publication No. 84 from the Immunology Division, University of Rochester Cancer Center. ’ To whom correspondence should be addressed at Box 704, University of Rochester Cancer Center, 601 Elmwood Ave., Rochester, NY 14642. 3 Abbreviations used: FL-&I, FL-coupled to Brucella abortus; HEM, Hepes-buffered medium; KLH, keyhole limpet hemocyanin; LPS, Escherichia coli lipopolysaccharide; immunoglobulin; TD, thymus dependent; TI, thymus independent.

PY - 1992/7

Y1 - 1992/7

N2 - The effects of priming on the susceptibility of B-cell subsets to tolerance induction have been tested in a model system in which anti-immunoglobulin (anti-Ig) has been employed as a surrogate for tolerogen. T-cell-depleted B cells were primed in vitro with fluorescein or trinitrophenylated Ficoll (a thymus-independent (TI) antigen) and then exposed overnight to anti-Ig to attempt to induce B-cell anergy. Primed cells were relatively resistant to this tolerance protocol and resistance was hapten specific. The dose response and kinetics suggested that this process was not due to receptor blockade or modulation, but was an active process. Moreover, this priming for resistance to tolerance was reproduced in vivo upon intraperitoneal treatment with haptenated Ficoll. Such in vivo priming for tolerance resistance was long-lasting and did not occur with a thymus-dependent priming protocol with fluoresceinated hemocyanin. These results are discussed in terms of TI priming to drive B cells into cycle and express novel functional and phenotypic properties.

AB - The effects of priming on the susceptibility of B-cell subsets to tolerance induction have been tested in a model system in which anti-immunoglobulin (anti-Ig) has been employed as a surrogate for tolerogen. T-cell-depleted B cells were primed in vitro with fluorescein or trinitrophenylated Ficoll (a thymus-independent (TI) antigen) and then exposed overnight to anti-Ig to attempt to induce B-cell anergy. Primed cells were relatively resistant to this tolerance protocol and resistance was hapten specific. The dose response and kinetics suggested that this process was not due to receptor blockade or modulation, but was an active process. Moreover, this priming for resistance to tolerance was reproduced in vivo upon intraperitoneal treatment with haptenated Ficoll. Such in vivo priming for tolerance resistance was long-lasting and did not occur with a thymus-dependent priming protocol with fluoresceinated hemocyanin. These results are discussed in terms of TI priming to drive B cells into cycle and express novel functional and phenotypic properties.

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Effect of priming with a thymus-independent antigen on susceptibility to B-Cell tolerance

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