Efficacy of resuscitation with fibrinogen concentrate and platelets in traumatic hemorrhage swine model

Wenjun Z. Martini*, Cassandra M. Rodriguez, Andrew P. Cap, Michael A. Dubick

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


BACKGROUND: This study compared the resuscitation effects of platelets and fibrinogen concentrate (FC) on coagulation and hemodynamics in pigs with traumatic hemorrhage and reduced platelet counts. METHODS: Thirty pigs (40 ± 3 kg) were anesthetized and catheterized with an apheresis catheter to remove platelets using the Haemonetics 9000 (Haemonetics, Braintree, MA). Afterward, a femur fracture was induced, followed by hemorrhage of 35% the estimated blood volume. Pigs were then randomized to be resuscitated with 5% human albumin (12.5 mL/kg), FC (250 mg/kg, 12.5 mL/kg), or platelets collected from apheresis (11.0 ± 0.5 mL/kg). Animals were monitored for 2 hours or until death. Blood samples were collected before (baseline [BL]) and after apheresis, after hemorrhage, and after resuscitation to assess changes in hemodynamics and coagulation using Rotem. RESULTS: No change in mean arterial pressure (MAP) or heart rate (HR) was observed by platelet apheresis. Hemorrhage reduced MAP to 57% ± 5% and elevated HR to 212% ± 20% of BL (both p < 0.05). Resuscitation with albumin, FC, or platelets did not revert MAP or HR to BL. Platelet counts were reduced by apheresis from BL 383 ± 20 × 103/μL to 141 ± 14 × 103/μL and were reduced further after resuscitation with albumin (88 ± 18 × 103/μL) or FC (97 ± 13 × 103/μL, all p < 0.05), but improved with platelet resuscitation (307 ± 24 × 103/μL). Fibrinogen concentration was reduced by apheresis from BL 225 ± 9 mg/dL to 194 ± 8 mg/dL, fell after albumin infusion (134 ± 11 mg/dL), increased to 269 ± 10 mg/dL after FC resuscitation (all p < 0.05), and was not affected by platelet resuscitation. Rotem α-angle decreased from 79 ± 2 degrees to 69 ± 1 degrees by apheresis and hemorrhage (p < 0.05), and recovered similarly by resuscitation with FC (87 ± 1 degrees) or platelets (78 ± 2 degrees), but not by albumin (63 ± 3 degrees). Similar responses were observed in Rotem maximum clot firmness. CONCLUSION: In this traumatic hemorrhage swine model, low-volume resuscitation with FC or platelets was similarly effective in restoring coagulation.

Original languageEnglish
Pages (from-to)S137-S145
JournalJournal of Trauma and Acute Care Surgery
Issue number2
StatePublished - Aug 2020
Externally publishedYes


  • Platelet apheresis
  • coagulation
  • fibrinogen concentrate
  • hemorrhage
  • swine


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